Nanoparticle-bacteria interaction study based on surface functionalization of nanometric structures.

• Grant number: 14/01241-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2014
• End date: August 07, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Mateus Borba Cardoso
• Grantee: Maiara Emer
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Campinas , SP, Brazil
• Associated scholarship(s): 15/22433-1 - Silica nanoparticles surface functionalization to improve penetration and treatment of biofilms, BE.EP.IC

Abstract

The present project aims to synthesize silica nanoparticles (SiO2NPs) andfunctionalize their surface with different chemical groups (mercapto, glycidyl,isocyanate, phenylethyl and amino) based on the objective of understanding how thedifferent functional groups favor the interaction of nanoparticles with the envelope ofdifferent bacteria. The functionalization of SiO2NPs with different functional groupsintends to select a system where the interaction nanoparticle-bacteria is enhanced. Thenanoparticles synthesized will be characterized by small-angle X-ray scattering (SAXS),nitrogen adsorption/desorption, transmission electron microscopy (TEM) and thermogravimetricanalysis (TGA). The efficiency of the modification and functionalization ofSiO2NPs will be monitored by Fourier transform infrared spectroscopy (FT-IR) as wellas by zeta potential. Incubation experiments with Escherichia coli (gram negative) and Staphylococcus aureus (gram positive) bacteria will be performed. Aliquots of the incubated material (nanoparticles + bacteria) will be analyzed by scanning electron microscopy (SEM) indifferent intervals of time. These experiments will be used to correlate the effect of thenanoparticles functionalization with the interaction mechanism between bacteria andnanoparticles.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

EMER, MAIARA; CARDOSO, MATEUS BORBA. Biomolecular corona formation: nature and bactericidal impact on surface-modified silica nanoparticles. JOURNAL OF MATERIALS CHEMISTRY B, v. 5, n. 40, p. 8052-8059, OCT 28 2017. (14/01241-4, 14/22322-2, 15/25406-5)