Synthesis of fluorescent nanoparticles using betalains

• Grant number: 15/25629-4
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2016
• End date: November 03, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Erick Leite Bastos
• Grantee: Arthur Bonfá Fernandes
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 19/25187-2 - Betalains as mediators in photoinduced electron transfer processes, BE.EP.DD

Abstract

The use of fluorescence in Cell Biology has contributed to expand the general understanding of the molecular basis of biological processes. Single molecule fluorescence, laser-induced fluorescence of biomolecules, tags and probes with non-linear optical properties, and fluorescent nanoparticles are state of the art in this field. In this PhD project, we propose to manufacture highly monodisperse fluorescent nanoparticles using betalains, a class of plant pigments, as reducing and stabilizing agents. The interaction of the nanoparticles obtained with animal cell lines will be studied by confocal microscopy experiments and the results will be analyzed considering structure-property relationships. Five groups of model-betalains will be semisynthesized and characterized (structure and photophysical and redox properties) for this study. Nanoparticles will be characterized by different techniques, including: electron microscopy, atomic force microscopy, X-ray scattering and X-rays photoelectron spectroscopy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REIS, JOEL S.; FERNANDES, ARTHUR B.; FREITAS-DORR, BARBARA C.; BASTOS, ERICK L.; STEFANI, HELIO A.. Oxazoline as acceptor moiety for excited-state intramolecular proton transfer. Tetrahedron, v. 74, n. 48, p. 6866-6872, NOV 29 2018. (14/22136-4, 15/25629-4, 14/10563-5, 17/24821-4, 16/02392-1, 16/21445-9)

FERNANDES, ARTHUR B.; PAVLIUK, V, MARIIA; PAUN, CRISTINA; CARVALHO, ALEXANDRINA C.; NOMURA, CASSIANA S.; LEWIN, ERIK; LINDBLAD, REBECKA; CAMARGO, PEDRO H. C.; SA, JACINTO; BASTOS, ERICK L.. Recoverable and Reusable Polymer Microbead-Supported Metal Nanocatalysts for Redox Chemical Transformations. ACS APPLIED NANO MATERIALS, v. 3, n. 2, p. 1722-1730, FEB 2020. (14/22136-4, 17/10346-2, 19/08450-1, 19/06391-8, 15/25629-4)

FREITAS-DORR, B. C.; MACHADO, C. O.; PINHEIRO, A. C.; FERNANDES, A. B.; DORR, F. A.; PINTO, E.; LOPES-FERREIRA, M.; ABDELLAH, M.; SA, J.; RUSSO, L. C.; et al. A metal-free blue chromophore derived from plant pigments. SCIENCE ADVANCES, v. 6, n. 14, APR 2020. (18/25842-8, 14/10563-5, 16/21445-9, 15/25629-4, 14/22136-4, 19/06391-8, 15/24760-0, 15/18474-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERNANDES, Arthur Bonfá. Use of betalainic compounds for nanoparticle synthesis and tuning of their characteristics. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.