Metabolic evaluation of the effects of replacement of fish oil by Plukenetia volubilis oil in juvenile rainbow trout Oncorhynchus mykiss

• Grant number: 15/23105-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2016
• End date: April 03, 2019
• Field of knowledge: Biological Sciences - Zoology - Physiology of Recent Groups
• Principal Investigator: Renata Guimarães Moreira Whitton
• Grantee: Bruno Tadeu Marotta Lima
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 17/23285-1 - Evaluation of oil from Camelina sativa in feeds for gilthead sea bream (Sparus aurata): effects on fish growth, tissue fatty acid composition, and gene expression, BE.EP.DR

Abstract

The fish oil (FO), particularly of marine origin, can be considered an excellent source of highly unsaturated fatty acids (HUFA) and long-chain essential to the physiological processes in animals and productive performance. However, due to the shortage of this product due to the reduction of natural marine stocks, this limited resource is considered insufficient for continued use. Certain teleosts freshwater have the capacity to synthesize HUFAs from essential fatty acids (EFA's) found in some vegetable oils. The volubilis Plukenetia, sacha inchi (SI) is an Amazonian oleaginous plant recently studied and produced in Brazil, with high content of EFA and ratio n3/n6 balanced, being a potential alternative to FO, however, it is necessary to investigate the benefits of its inclusion in diets for teleost. Based on the above, the objective of this project is to evaluate the metabolic responses replacing the FO by different levels of inclusion of sacha inchi oil (0, 40, 60 and 100%) in juvenile diet Oncorhynchus mykiss. Will be assessed energy substrates, enzymatic pathways involved in the synthesis of fatty acids (FA) and the FA of body balance in offering this oil. The experiment will be conducted in two stages, the first will be evaluated acceptance of diets for trout and confirm the proposed inclusion levels. In the next step 240 juveniles are distributed into eight tanks composing the four treatments during 12 weeks. Biometrics will be held every 14 days to evaluate the growth and, at 45 and 90 days will be collected eight fish per treatment for analysis of body composition, and eight fish to assess somatic indices, biochemical variables, lipid metabolism, and enzymes elongation and desaturation of the FA.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAROTTA LIMA, BRUNO TADEU; TAKAHASHI, NEUZA SUMICO; TABATA, YARA AIKO; HATTORI, RICARDO SHOHEI; DA SILVA RIBEIRO, GRISTLELE; MOREIRA, RENATA GUIMARAES. Balanced omega-3 and-6 vegetable oil of Amazonian sacha inchi act as LC-PUFA precursors in rainbow trout juveniles: Effects on growth and fatty acid biosynthesis. Aquaculture, v. 509, p. 236-245, JUL 15 2019. (15/23105-8)