Canine mammary tumor characterization (tubular carcinoma) with emphasis on extracellular matrix

• Grant number: 15/25815-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2016
• End date: March 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Phelipe Oliveira Favaron
• Grantee: Marcella Giancoli Kato Cano da Silva
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The occurrence of breast tumors in dogs has increased significantly in recent years, which means that further studies may be needed to understand the morphological and physiological events that lead to the development / tumor growth and tissue changes, contributing to the elucidation of new treatments . In addition, many commonalities relating to the biological process of developing these tumors are common in dogs and humans, thereby serving the dog, as an interesting experimental model. However, the literature shows that tumors vary widely according to their degree of malignity, as soon require specific and detailed characterization and identification. The ratings should be made based on histopathological analysis, which are differentiated the tubular, papillary and solid for carcinoma types, according to the cell type and tissue arrangement. Since the literature is still rather scarce on this type of analysis, this project aims to characterize breast tumors (tubular carcinoma) in dogs, based on analyzes histopathological, and also in immunohistochemical analysis (expression of markers involved with epithelial-mesenchymal transition, cell cycle, growth factor and hormone receptors). In addition, special attention will be given the structural changes in the extracellular matrix of tumors as well as in its collagen composition. The analysis will also be conducted in healthy mammary gland samples in order to obtain parameters that can be compared with the results obtained with tumors, as well as understand the changes that occur during carcinogenesis. The results of this project may contribute significantly to the knowledge of the phenotypic characteristics of the tubular carcinoma in dogs as regards the expression of markers, as well as estrogen and progesterone receptors, beyond knowing about the changes that occur in the extracellular matrix during tumor development.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORGHES, JESSICA; DA SILVA, MARCELLA GIANCOLI KATO CANO; GUIMARAES, KATIA DE OLIVEIRA PIMENTA; MARIO, LARA CAROLINA; DA ANUNCIACAO, ADRIANA RAQUEL DE ALMEIDA; RABELO, ANA CAROLINA SILVEIRA; HAYASHI, RAFAEL GONCALVES; LIMA, MARIANA FERREIRA; MIGLINO, MARIA ANGELICA; FAVARON, PHELIPE OLIVEIRA; et al. Evaluation of immunohistopathological profile of tubular and solid canine mammary carcinomas. Research in Veterinary Science, v. 136, p. 119-126, MAY 2021. (15/25815-2)