Evaluation of a. salina as a toxicity alternative method in antibiotics used against tuberculosis

• Grant number: 15/24632-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2016
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Fernando Rogério Pavan
• Grantee: Ana Carolina Loiola Pereira
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 13/14957-5 - Research potential against tuberculosis of a new class of furoxan compounds and nanostructured compounds of the ruthenium(II) and copper (II), AP.JP

Abstract

Preclinical toxicology tests are routinely performed in mice. However, due to the high cost and ethical issues related to the use of animals, there is currently a growing need and incentive to the study and development of alternative methods for the reduction, refinement of animal testing or even replacing it with other models. Among the alternatives, there is the test with Artemia salina.The Artemia salina l. (Artemiidae), is a marine microcrustaceo used in laboratory tests for assessing the toxicity of chemicals from the determination of median lethal concentration (LD50). Tests with A. salina already demonstrated to be reliable and possess great potential in relation to predictive toxicity, because of its simplicity, speed and cost-benefit tests lethality with this organism has been considered very promising. Thus, this project aims to evaluate the effectiveness of the test with A. salina in predicting the toxicity of drugs used reference in therapy against tuberculosis, compared to toxicity values in in vitro assays conducted in normal cell lineages and still correlating these results with LD50 values obtained in tests for acute toxicity in mice. In addition, we know that in vitro assays have many limitations, because they produce a high rate of positive data irrelevant and possible false negative data due to the absence of metabolism in these assays. From this, to move closer to the human body, the experiment will be carried out with the untreated and treated with S9 fraction, which is an angle inclination of rat liver cells.