Potential evaluation of nanostructured lipid systems for the incorporation of silver (I) complexes apply in the study against Mycobacterium tuberculosis

• Grant number: 16/10449-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2016
• Effective date (End): July 31, 2017
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Patrícia Bento da Silva
• Grantee: Rachel Temperani Amaral Machado
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Tuberculosis (TB), the leading cause of death from curable infectious diseases, continues to grow in absolute numbers and is more present today than at any other stage of humanity. No specific drug against this disease has been developed since 1960 and the emergence of multidrug-resistant TB cases makes the ineffectiveness of current treatment. The íon Ag (I) has a tendency to form complexes with other molecules, forming coordination compounds with different activities, among them antimicrobial. Nanostructured lipid systems, such as microemulsions (MEs) have gained prominence in the field of pharmaceutical sciences by reason of improve the solubility and stability of pharmaceuticals, in addition to providing prolonged action and can carrier substances with different degrees of hydrophilicity / lipophilicity in the same formulation. This work aims to develop and characterize MEs containing coordination compounds of silver (I) and carry out in vitro biological assays against Mycobacterium tuberculosis. Complexes of Ag (I) were developed and characterized by Segura et al.. Since such compounds showed low selectivity index (SI) and cytotoxicity, compromising their biopharmaceutical properties and made it unfeasible to in vivo assay, such compounds will be incorporated into in this project MEs, which are characterized by dynamic light scattering, polarized light microscopy and zeta potential and. As for in vitro biological assays will be determined the minimum inhibitory concentration (MIC) against Mtb H37Rv ATCC 27294 e the cytotoxicity index (IC50) against Vero (ATCC® CCL - 81), J774A.1 (ATCC® TIB-67), and MRC-5 (ATCC® CCL - 171) cell lines. The toxicity will be determined an alternative model (Artemia salina L.). It is expected therefore to obtain a nanostructural level system that can optimize the study of Ag(I) complexes against M. tuberculosis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, PATRICIA BENTO; AMARAL MACHADO, RACHEL TEMPERANI; PIRONI, ANDRESSA MARIA; ALVES, RENATA CAROLINA; DE ARAUJO, PATRICIA ROCHA; DRAGALZEW, AMANDA CUTRIM; DALBERTO, INGRID; CHORILLI, MARLUS. Recent Advances in the Use of Metallic Nanoparticles with Antitumoral Action - Review. Current Medicinal Chemistry, v. 26, n. 12, p. 2108-2146, 2019. (16/10449-3)