EVALUATION OF THE ROLE OF GENES ENCODING ACC SINTHASE IN THE DEVELOPMENT OF SUGARCANE PLANTS

• Grant number: 15/23789-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2016
• End date: July 31, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Marcelo Menossi Teixeira
• Grantee: Lucia Mattiello
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/15576-5 - Molecular approaches to study the relationship between ethylene and sugarcane ripening, AP.BIOEN.TEM
• Associated scholarship(s): 17/09817-0 - Sugarcane genome editing using CRISPR/Cas9 for targeted mutagenesis of ethylene biosynthesis, BE.EP.PD

Abstract

Understanding sugarcane ripening control is an interesting scientific challenge. Ethylene is a key component for maturation on several plant species. However, in sugarcane, a plant with unique sucrose accumulation characteristics, the role of ethylene remains elusive. Our hypothesis is that ethylene is a key hormone for several sugarcane developmental steps. This project has as goal the production of transgenic sugarcane plants silenced for the genes coding the enzyme 1-aminocyclopropane-1-carboxylic acid synthase (ACS) using interference RNA and evaluate the physiological and biometrical status of the transgenic plants. In order to achieve this goal we propose (a) production of a hairpin-based silencing cassette; (b) genetic transformation of sugarcane plants; (c) evaluation of the biometric status through quantification of green leaves, dry leaves, number of tillers, internodes growth and internodes diameter; (d) evaluation of physiological status through quantification of photosynthetic activity, chlorophyll content, carbon isotopic discrimination, sugar and ethylene quantification. The comprehension of the developmental implications of the reduced content of the ACS on sugarcane will allow the determination of the role of the ethylene on sugar accumulation and in other processes such as biomass production and stress response.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, IVONE DE BEM; NUNES, RHEWTER; MATTIELLO, LUCIA; BARROS-RIBEIRO, STELA; DE SOUZA, ISABELA PAVANELLI; GUEDES COELHO, ALEXANDRE SIQUEIRA; COLLEVATTI, ROSANE GARCIA. Research and partnership in studies of sugarcane using molecular markers: a scientometric approach. SCIENTOMETRICS, v. 119, n. 1, p. 335-355, APR 2019. (15/23789-4)

GUIDELLI, GIOVANNA, V; MATTIELLO, LUCIA; GALLINARI, RAFAEL H.; DE LUCCA, PAULO CEZAR; MENOSSI, MARCELO. pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 2, p. 5-pg., 2018-04-01. (15/08659-7, 13/15576-5, 15/23789-4, 14/26521-0)

GUIDELLI, V, GIOVANNA; MATTIELLO, LUCIA; GALLINARI, RAFAEL H.; DE LUCCA, PAULO CEZAR; MENOSSI, MARCELO. pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 2, p. 450-454, APR-JUN 2018. (15/08659-7, 15/23789-4, 13/15576-5, 14/26521-0)