Characterization of ENC1 (NRP/B) isoforms and identification of human glioblastoma-penetrating peptide

• Grant number: 16/01626-9
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: May 01, 2016
• End date: September 30, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Mari Cleide Sogayar
• Grantee: Túlio Felipe Pereira
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 16/24797-3 - Identification and validation of the binding ligands corresponding to two human glioblastoma penetrating peptides, BE.EP.DD

Abstract

Gliomas are the most common form of primary intracranial malignancy. High-grade gliomas, also called glioblastoma multiforme, are the most frequently encountered, carrying the worst prognosis (12 months life expectancy after diagnosis). Therapy of brain tumor has been limited by the lack of effective methods for drug delivery, therefore, it is mandatory to seek for new approches in gliomas therapy development. A previous study from our group revealed that over expression of the Ectodermal-Neural Cortex 1 (ENC 1) cDNA in rat glioma cells (C6/ST1) resulted in a significative reduction of soft agar growth and in vivo tumorigenic potencial. ENC1 has two protein isoforms, namely: a 67 kDa (Longer) and a 57 kDa (Shorter). The shorter one was identified in primary neurons, however it has not been characterized yet. Peptides and proteins that can be internalized by cells have been the subject of intensive study in recent years, since translocation across the cell membrane is critical for drug delivery and gene therapy. This project has two main objectives. Firstly, to characterize the potential of ENC1 isoforms to suppress the tumorigenic phenotype and to identify Glioblastoma-Penetrating Peptides (GPP). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, TULIO FELIPE; LEVIN, GABRIEL; DEOCESANO-PEREIRA, CARLOS; CAODAGLIO, AMANDA SCHIERSNER; FUJITA, ANDRE; TONSO, ALDO; SOGAYAR, MARI CLEIDE. Fluorescence-based method is more accurate than counting-based methods for plotting growth curves of adherent cells. BMC RESEARCH NOTES, v. 13, n. 1, p. 7-pg., 2020-02-04. (16/05311-2, 16/01626-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PEREIRA, Túlio Felipe. Role of Ectodermal-neural cortex 1 protein in human glioma progression, identification of a peptide internalized by human glioblastoma cells and development of an alternative method to generate growth curves of adherent cultures. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.