Prophylaxis of sheep worms using integrated crop-livestock system (ICL)

• Grant number: 15/25718-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2016
• End date: May 31, 2019
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Alessandro Francisco Talamini Do Amarante
• Grantee: Fabiana Alves de Almeida
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The objective of this research is to assess whether the integrated crop-livestock systems (ICL) involving corn, pigeon pea and oats in consortium with marandugrass, can be used in the control of worms in sheep. The ICL systems will be evaluated: (1) corn + palisadegrass + oats spread out manually; (2) corn + marandugrass + pigeon pea + oats spread out manually; (3) corn + marandugrass + oats on the line; (4) corn + marandugrass + pigeon pea + oats in line. Will be evaluated two experimental cycles (2015/ 2016 and 2016/ 2017) and in each cycle 48 male lambs (n = 12) crossbred Dorper x Santa Ines with 90 days of age and 25 kg body weight will be used. Rotational grazing system (12 paddocks per treatment) with 3 days of occupation and 33 days of rest will be employed. At the end of the day the lambs will be collected for a covered shed with a dirt floor lined with bark dried coffee, in which the test-lambs of each treatment will be allocated in the same pens and will be supplemented with concentrate + silage from the same treatment. In order to check if the pasture is free from contamination by gastrointestinal parasites before the animals enter, will be three collections grazing in each paddock with an interval of nine days between them. At the beginning the lambs trial will be treated with monepantel (2.5 mg / kg of body weight (BW), Zolvix®, Novartis Animal Health) to eliminate infection Trichostrongylideos. Every 12 days, the animals will be weighed to estimate weight gain and fecal samples from the rectum of animals will be collected to make the eggs per gram of feces (EPG) and composite fecal cultures, blood will also be collected to verify the packed cell volume (PCV). Every nine days samples of the pasture will be collected to check their contamination by infective larvae (L3) of gastrointestinal parasites. After 72 days the experimental lambs are slaughtered at a commercial abattoir. The abomasum and small and large intestine are collected for recovery of worms and subsequent identification and counting thereof. It is expected to validate the ICP system as a method of control of sheep worms. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JOSÉ GABRIEL G. LINS; FABIANA A. ALMEIDA; ALESSANDRO F.T. AMARANTE. Variáveis hematológicas de cordeiros lactentes Santa Inês e Ile de France: influência da infecção por Haemonchus contortus. Pesquisa Veterinária Brasileira, v. 40, n. 11, p. 882-891, 2021-01-18. (15/25718-7)

DE ALMEIDA, FABIANA ALVES; BASSETTO, CESAR CRISTIANO; VENDRAME AMARANTE, MONICA REGINA; ALEXANDRE DE ALBUQUERQUE, ANA CLAUDIA; COSTA STARLING, RENAN ZAPPAVIGNA; TALAMINI DO AMARANTE, ALESSANDRO FRANCISCO. Helminth infections and hybridization between Haemonchus contortus and Haemonchus placei in sheep from Santana do Livramento, Brazil. Revista Brasileira de Parasitologia Veterinária, v. 27, n. 3, p. 280-288, JUL-SEP 2018. (15/25718-7)

BASSETTO, CESAR C.; ALMEIDA, FABIANA A.; NEWLANDS, GEORGE F. J.; SMITH, W. DAVID; AMARANTE, ALESSANDRO F. T.. Repeated vaccination against Haemonchus contortus provides continuous protection to young grazing sheep. Veterinary Parasitology, v. 287, NOV 2020. (15/00221-2, 15/25718-7)

BASSETTO, C. C.; ALMEIDA, F. A.; NEWLANDS, G. F. J.; SMITH, W. D.; CASTILHOS, A. M.; FERNANDES, S.; SIQUEIRA, E. R.; AMARANTE, A. F. T.. Trials with the Haemonchus vaccine, Barbervax (R), in ewes and lambs in a tropical environment: Nutrient supplementation improves protection in periparturient ewes. Veterinary Parasitology, v. 264, p. 52-57, DEC 15 2018. (15/00221-2, 15/25718-7)

ALMEIDA, FABIANA A.; ALBUQUERQUE, ANA CLAUDIA A.; BASSETTO, CESAR C.; STARLING, RENAN Z. C.; LINS, JOSE GABRIEL G.; AMARANTE, ALESSANDRO F. T.. Long spelling periods are required for pasture to become free of contamination by infective larvae of Haemonchus contortus in a humid subtropical climate of Sao Paulo state, Brazil. Veterinary Parasitology, v. 279, MAR 2020. (15/25718-7, 15/00221-2)

SABIAO DE TOLEDO PIZA, MARINA LAIS; DE ALMEIDA, FABIANA ALVES; PARIZ, CRISTIANO MAGALHAES; COSTA, CINIRO; TALAMINI DO AMARANTE, ALESSANDRO FRANCISCO. Comparison of two methods for the quantification of gastrointestinal nematode infective larvae from pasture. SEMINA-CIENCIAS AGRARIAS, v. 40, n. 2, p. 713-722, MAR-APR 2019. (13/13702-3, 15/25413-1, 15/25718-7)

ALBUQUERQUE, ANA CLAUDIA A.; BASSETTO, CESAR CRISTIANO; ALMEIDA, FABIANA A.; HILDERSLEY, KATIE A.; MCNEILLY, TOM N.; BRITTON, COLLETTE; AMARANTE, ALESSANDRO F. T.. Differences in immune responses to Haemonchus contortus infection in the susceptible Ile de France and the resistant Santa Ines sheep under different anthelmintic treatments regimens. VETERINARY RESEARCH, v. 50, n. 1, NOV 29 2019. (15/25718-7, 15/00221-2)

ALBUQUERQUE, ANA CLAUDIA A.; ALMEIDA, FABIANA A.; BASSETTO, CESAR C.; AMARANTE, ALESSANDRO F. T.. Influence of targeted selective anthelmintic treatment on the productive performance of wool and hair lambs naturally infected with gastrointestinal nematodes in Brazil. VETERINARY RESEARCH COMMUNICATIONS, v. N/A, p. 10-pg., 2023-01-03. (15/00221-2, 15/25718-7)

ALEXANDRE DE ALBUQUERQUE, ANA CLAUDIA; BASSETTO, CESAR CRISTIANO; DE ALMEIDA, FABIANA ALVES; AMARANTE, ALESSANDRO F. T.. Development of Haemonchus contortus resistance in sheep under suppressive or targeted selective treatment with monepantel. Veterinary Parasitology, v. 246, p. 112-117, NOV 15 2017. (15/00221-2, 15/25718-7)

LINS, JOSE GABRIEL G.; ALMEIDA, FABIANA A.; ALBUQUERQUE, ANA CLAUDIA A.; BRITTON, COLLETTE; AMARANTE, ALESSANDRO F. T.. Early-onset immune response to Haemonchus contortus infection in resistant Santa Ines suckling lambs compared with susceptible Ile de France. Veterinary Parasitology, v. 307-308, p. 10-pg., 2022-07-01. (21/03479-1, 15/25718-7)

DE ALMEIDA, FABIANA ALVES; BASSETTO, CESAR CRISTIANO; VENDRAME AMARANTE, MONICA REGINA; ALEXANDRE DE ALBUQUERQUE, ANA CLAUDIA; COSTA STARLING, RENAN ZAPPAVIGNA; TALAMINI DO AMARANTE, ALESSANDRO FRANCISCO. Helminth infections and hybridization between Haemonchus contortus and Haemonchus placei in sheep from Santana do Livramento, Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 27, n. 3, p. 9-pg., 2018-07-01. (15/25718-7)

LINS, JOSE GABRIEL G.; ALMEIDA, FABIANA A.; AMARANTE, ALESSANDRO F. T.. Haematological variables of Santa Ines and Ile de France lambs: Influence of Haemonchus contortus infection. Pesquisa Veterinária Brasileira, v. 40, n. 11, p. 10-pg., 2020-11-01. (15/25718-7)

ALMEIDA, FABIANA A.; PIZA, MARINA L. S. T.; BASSETTO, CESAR C.; STARLING, RENAN Z. C.; ALBUQUERQUE, ANA CLAUDIA A.; PROTES, VERENA M.; PARIZ, CRISTIANO M.; CASTILHOS, ANDRE M.; COSTA, CINIRO; AMARANTE, ALESSANDRO F. T.. Infection with gastrointestinal nematodes in lambs in different integrated crop-livestock systems (ICL). Small Ruminant Research, v. 166, p. 66-72, SEP 2018. (13/13702-3, 15/25413-1, 13/23853-9, 15/25718-7)

PARIZ, CRISTIANO MAGALHAES; COSTA, NIDIA RAQUEL; COSTA, CINIRO; COSTA CRUSCIOL, CARLOS ALEXANDRE; DE CASTILHOS, ANDRE MICHEL; DE LIMA MEIRELLES, PAULO ROBERTO; CALONEGO, JULIANO CARLOS; ANDREOTTI, MARCELO; DE SOUZA, DANIEL MARTINS; CRUZ, IGOR VILELA; et al. An Innovative Corn to Silage-Grass-Legume Intercropping System With Oversown Black Oat and Soybean to Silage in Succession for the Improvement of Nutrient Cycling. FRONTIERS IN SUSTAINABLE FOOD SYSTEMS, v. 4, NOV 24 2020. (15/25413-1, 14/12950-6, 17/05044-7, 14/14935-4, 13/23853-9, 14/21772-4, 13/13702-3, 15/25718-7)

ALBUQUERQUE, A. C. A.; ALMEIDA, F. A.; BASSETTO, C. C.; LINS, J. G. G.; AMARANTE, A. F. T.. Influence of breed and parasite challenge on the immune response to naturally acquired intestinal nematode infection in sheep. JOURNAL OF HELMINTHOLOGY, v. 96, p. 9-pg., 2022-04-18. (15/25718-7, 15/00221-2)

RENAN ZAPPAVIGNA COSTA STARLING; FABIANA ALVES DE ALMEIDA; MARCUS VINÍCIUS GONÇALVES VIANA; ANDRÉ MICHEL DE CASTILHOS; ALESSANDRO FRANCISCO TALAMINI DO AMARANTE. Prejuízos causados pelas infecções por nematoides gastrintestinais em cordeiros Dorper submetidos a dois manejos nutricionais. Revista Brasileira de Parasitologia Veterinária, v. 28, n. 4, p. 652-660, 2019-11-04. (15/14751-3, 15/25718-7)

COSTA STARLING, RENAN ZAPPAVIGNA; DE ALMEIDA, FABIANA ALVES; GONCALVES VIANA, MARCUS VINICIUS; DE CASTILHOS, ANDRE MICHEL; TALAMINI DO AMARANTE, ALESSANDRO FRANCISCO. Losses caused by gastrointestinal nematode infections in Dorper lambs under two nutritional status. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 28, n. 4, p. 9-pg., 2019-10-01. (15/25718-7, 15/14751-3)