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Characterization of the DEAD-box RNA helicase CC1478 in Caulobacter crescentus and its regulation

Grant number: 16/06378-3
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2016
Effective date (End): July 31, 2018
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal researcher:Marilis Do Valle Marques
Grantee:Rodolfo Alvarenga Ribeiro
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:14/04046-8 - Regulatory networks of bacterial stress response, AP.TEM

Abstract

DEAD-box RNA helicases are enzymes that have a role in several important biologicalprocesses, such as nucleic acids degradation or stabilization, assembly of riboproteiccomplexes and ribosome biogenesis. Caulobacter crescentus is an oligotrophic bacteriumbelonging to the ±-proteobacteria group, which has four genes encoding RNA helicases of theDEAD-box family. Previous work has shown that one of these enzymes, encoded by CC1478,is a functional RNA helicase, but has an atypical carboxi-terminal tail, probably generated bygenetic alterations conserved in the Alpha-proteobacteria.This work aims to characterize theregulation of the helicase CC1478, determining its transcriptional or post-transcriptionalregulation, and evaluate the phylogenetic information to assess the evolutive origin of thisalteration. The importance of this C-terminal domain for the enzyme function will be assessedby deletion of site-directed mutagenesis. The protein expression in the cell cycle and in stressresponse, and its participation in protein complexes, such as the ribosome or RNAdegradosome will be studied.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, LARISSA G.; LORENZETTI, ALAN P. R.; RIBEIRO, RODOLFO A.; ALVES, INGRID R.; LEADEN, LAURA; GALHARDO, RODRIGO S.; KOIDE, TIE; MARQUES, MARILIS V. OxyR and the hydrogen peroxide stress response in Caulobacter crescentus. Gene, v. 700, p. 70-84, JUN 5 2019. Web of Science Citations: 0.
ASSIS, NADINE G.; RIBEIRO, RODOLFO A.; DA SILVA, LARISSA G.; VICENTE, ALEXANDRE M.; HUG, ISABELLE; MARQUES, MARILIS V. Identification of Hfq-binding RNAs in Caulobacter crescentus. RNA BIOLOGY, v. 16, n. 6 MAR 2019. Web of Science Citations: 0.
LEADEN, LAURA; SILVA, LARISSA G.; RIBEIRO, RODOLFO A.; DOS SANTOS, NAARA M.; LORENZETTI, ALAN P. R.; ALEGRIA, THIAGO G. P.; SCHULZ, MARIANE L.; MEDEIROS, MARISA H. G.; KOIDE, TIE; MARQUES, V, MARILIS. Iron Deficiency Generates Oxidative Stress and Activation of the SOS Response in Caulobacter crescentus. FRONTIERS IN MICROBIOLOGY, v. 9, AUG 28 2018. Web of Science Citations: 5.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.