DEAD-box RNA helicases are enzymes that have a role in several important biologicalprocesses, such as nucleic acids degradation or stabilization, assembly of riboproteiccomplexes and ribosome biogenesis. Caulobacter crescentus is an oligotrophic bacteriumbelonging to the ±-proteobacteria group, which has four genes encoding RNA helicases of theDEAD-box family. Previous work has shown that one of these enzymes, encoded by CC1478,is a functional RNA helicase, but has an atypical carboxi-terminal tail, probably generated bygenetic alterations conserved in the Alpha-proteobacteria.This work aims to characterize theregulation of the helicase CC1478, determining its transcriptional or post-transcriptionalregulation, and evaluate the phylogenetic information to assess the evolutive origin of thisalteration. The importance of this C-terminal domain for the enzyme function will be assessedby deletion of site-directed mutagenesis. The protein expression in the cell cycle and in stressresponse, and its participation in protein complexes, such as the ribosome or RNAdegradosome will be studied.
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