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Development of transgenic sugarcane more resistant to Sphenophorus levis

Grant number: 15/10855-9
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2016
Effective date (End): May 31, 2020
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal researcher:Flavio Henrique da Silva
Grantee:Chakravarthi Mohan
Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Associated scholarship(s):18/11544-5 - CRISPR/Cas9 based targeted insertion of cystatins in a pre-characterized locus of sugarcane, BE.EP.PD

Abstract

The beetle larvae Sphenophorus levis are one of the major pests of sugarcane plantations in the state of São Paulo, and so far, no method of control has been effective against the insect. Within this context, control measures are necessary to minimize the damage caused by this pest. This project envisages in the development of transgenic sugarcane more resistant to Sphenophorus levis. Initially, the transcriptome data of S. levis obtained from studies at the Laboratory of Molecular Biology, Department of Genetics and Evolution, Federal University of São Carlos, would be analyzed to identify target genes for gene silencing by RNAi. The objectives of the study are to identify target genes from the transcriptome data, develop vector constructs with target genes to be silenced, to generate transgenic sugarcane through both Biolistics and Agrobacterium mediated transformation methods and to evaluate the transgenics for S. levis resistance. The targets preliminarily studied at the laboratory which includes an encoder of a serpin and a V-ATPase gene will also be used for sugarcane genetic transformation.

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MOHAN, CHAKRAVARTHI; EASTERLING, MONA; YAU, YUAN-YEU. ene Editing Technologies for Sugarcane Improvement: Opportunities and Limitation. SUGAR TECH, v. 24, n. 1, SI OCT 2021. Web of Science Citations: 0.
MOHAN, CHAKRAVARTHI; SHIBAO, PRISCILA YUMI TANAKA; DE PAULA, FERNANDO FONSECA PEREIRA; TOYAMA, DANYELLE; VIEIRA, MARCOS ANTONIO SANCHES; FIGUEIRA, ANTONIO; SCOTTON, DANIELLE; SOARES-COSTA, ANDREA; HENRIQUE-SILVA, FLAVIO. hRNAi-mediated knock-down of Sphenophorus levis V-ATPase E in transgenic sugarcane (Saccharum spp interspecific hybrid) affects the insect growth and survival. Plant Cell Reports, v. 40, n. 3, p. 507-516, MAR 2021. Web of Science Citations: 0.
TANAKA SHIBAO, PRISCILA YUMI; SANTOS-JUNIOR, CELIO DIAS; SANTIAGO, ADELITA CAROLINA; MOHAN, CHAKRAVARTHI; MIGUEL, MARIANA CARDOSO; TOYAMA, DANYELLE; SANCHES VIEIRA, MARCOS ANTONIO; NARAYANAN, SUBRAMONIAN; FIGUEIRA, ANTONIO; CARMONA, ADRIANA K.; SCHIERMEYER, ANDREAS; SOARES-COSTA, ANDREA; HENRIQUE-SILVA, FLAVIO. Sugarcane cystatins: From discovery to biotechnological applications. International Journal of Biological Macromolecules, v. 167, p. 676-686, JAN 15 2021. Web of Science Citations: 0.
KARASAWA, MARINES MARLI GNIECH; MOHAN, CHAKRAVARTHI. Fruits as Prospective Reserves of bioactive Compounds: A Review. NATURAL PRODUCTS AND BIOPROSPECTING, v. 8, n. 5, p. 335-346, OCT 2018. Web of Science Citations: 5.
MOHAN, CHAKRAVARTHI; JAYANARAYANAN, ASHWIN NARAYAN; NARAYANAN, SUBRAMONIAN. Construction of a novel synthetic root-specific promoter and its characterization in transgenic tobacco plants. 3 BIOTECH, v. 7, JUL 10 2017. Web of Science Citations: 3.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.