Characterization of expression profiles of milk proteins in healthy and subclinical mastitis affected dairy buffaloes

• Grant number: 16/10526-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 01, 2016
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Principal Investigator: Humberto Tonhati
• Grantee: Fernanda Tanamati
• Supervisor: Jeremy Taylor
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Institution abroad: University of Missouri, Columbia (UM), United States
• Associated to the scholarship: 14/19321-4 - Study of the expression of genes related to mastitis in dairy buffaloes, BP.DR

Abstract

Buffalo milk has a high total solids content, mainly protein and fat, which assure high yield in its processing. However, mastitis, an inflammatory disease of the mammary gland, affects the quality and quantity of milk produced. In this context, new scientific approaches such as proteomics, have been widely used in animal research in general, but mainly in attempting to understand the nature of the disease in dairy animals. The aim of this study is to evaluate the proteins that are present in buffalo milk with, and without, subclinical mastitis using a proteomic approach with the goal of identifying potential biomarkers for infection. For this purpose, samples of milk from buffaloes diagnosed with mastitis and healthy animals will be collected for extraction and quantitation of proteins, which will subsequently be digested with trypsin. Differential expression to be verified by a mass spectrometer coupled to a nanoflow chromatography system (LC-MS/MS). The comparative and quantitative analyzes of the samples will be performed using the spectral counting method. The results will be important to further understand the behavior of the disease in the mammary gland and therefore may aid in early diagnosis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TANAMATI, FERNANDA; TAYLOR, JEREMY F.; BEHURA, SUSANTA K.; SANTOS, DANIEL J. A.; STAFUZZA, NEDENIA B.; ANDRADE, WILLIAN B. F.; GASPARINO, ELIANE; TONHATI, HUMBERTO. Short communication: Characterization of the milk protein expression profiles in dairy buffaloes with and without subclinical mastitis. JOURNAL OF DAIRY SCIENCE, v. 103, n. 3, p. 2677-2684, MAR 2020. (16/10526-8, 14/19321-4, 14/25309-7)