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Metabolic engineering and identification of quantitative traits loci related to HMF tolerance of Saccharomyces cerevisiae strain

Grant number: 15/06677-8
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): June 01, 2016
Effective date (End): September 30, 2019
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Gonçalo Amarante Guimarães Pereira
Grantee:Fellipe da Silveira Bezerra de Mello
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The urge to replace fossil fuels due to the political, economic and environmental issues have motivated research and development of renewable energy, with bioethanol being one of the main alternatives. Yeasts used in both processes - first and second generation - are responsible for a significant part in the viability of economic models. In the industrial environment, microorganisms are subjected to a variety of stresses that adversely affect production. Thus, tolerate such conditions is essential to obtain satisfactory yields. In the production process of second generation, besides environmental factors, inhibitors generated during the biomass processing constitute a barrier to the metabolism of yeasts, including weak acids, phenolic compounds or furan derivatives such as 5-hydroxymethylfurfural (HMF). In this work, it is proposed the detailed study of FMY001 strain, identified previously as tolerant to high HMF concentration. The genetic basis of its strength will be understood from the detailed study of Quantitative Trait Loci (QTL) related to the observed phenotype. For this, it will be used a flow cytometry-based method to obtain a high number of segregants derivate from FMY001 strain (or hybrid FMY001 x S288c). These will be individually characterized by means of high-throughput screening methods and sequenced for further QTL mapping. The cofactor metabolism - NAD (P) +, NAD (P) H - will also be evaluated by metabolic engineering techniques. Thus, understanding the genetic mechanisms related to the ability of FMY001 strain to tolerate the HMF will contribute significantly to the continued development of more productive strains. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARSOTTINI, MARIO R. O.; PIRES, BARBARA A.; VIEIRA, MARIA L.; PEREIRA, JOSE G. C.; COSTA, PAULO C. S.; SANITA, JAQUELINE; CORADINI, ALESSANDRO; MELLO, FELLIPE; MARSCHALK, CIDNEI; SILVA, EDER M.; PASCHOAL, DANIELE; FIGUEIRA, ANTONIO; RODRIGUES, FABIO H. S.; CORDEIRO, ARTUR T.; MIRANDA, PAULO C. M. L.; OLIVEIRA, PAULO S. L.; SFORCA, MAURICIO L.; CARAZZOLLE, MARCELO F.; ROCCO, SILVANA A.; PEREIRA, GONCALO A. G. Synthesis and testing of novel alternative oxidase (AOX) inhibitors with antifungal activity against Moniliophthora perniciosa (Stahel), the causal agent of witches' broom disease of cocoa, and other phytopathogens. Pest Management Science, v. 75, n. 5, p. 1295-1303, MAY 2019. Web of Science Citations: 1.
NAGAMATSU, SHEILA TIEMI; TEIXEIRA, GLEIDSON SILVA; BEZERRA DE MELLO, FELLIPE DA SILVEIRA; GALVAO TIZEI, PEDRO AUGUSTO; GRICHOSWSKI NAKAGAWA, BRUNA TATSUE; DE CARVALHO, LUCAS MIGUEL; GUIMARAES PEREIRA, GONCALO AMARANTE; CARAZZOLLE, MARCELO FALSARELLA. Genome Assembly of a Highly Aldehyde-Resistant Saccharomyces cerevisiae SA1-Derived Industrial Strain. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 8, n. 13 MAR 2019. Web of Science Citations: 1.
BEZERRA DE MELLO, FELLIPE DA SILVEIRA; VENEGA CORADINI, ALESSANDRO LUIS; GALVAO TIZEI, PEDRO AUGUSTO; CARAZZOLLE, MARCELO FALSARELLA; GUIMARAES PEREIRA, GONCALO AMARANTE; TEIXEIRA, GLEIDSON SILVA. Static microplate fermentation and automated growth analysis approaches identified a highly-aldehyde resistant Saccharomyces cerevisiae strain. BIOMASS & BIOENERGY, v. 120, p. 49-58, JAN 2019. Web of Science Citations: 4.

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