Identification of the partner proteins of Yes-Associated Protein (YAP) in mammary gland epithelial cells exposed to reconstituted basement membrane

• Grant number: 16/09561-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2016
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Alexandre Bruni Cardoso
• Grantee: Antonio Carlos Manucci Pereira Júnior
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 14/10492-0 - Hippo-YAP as a converging pathway for biochemical and mechanical signals from the extracellular matrix during mammary gland morphogenesis and breast cancer progression, AP.JP

Abstract

Extracellular matrix (ECM)-signaling is crucial for determination of cell fate and behavior, during mammary gland morphogenesis and homeostasis. However, little is known about the molecular mechanisms that regulate these processes. The Hippo pathway, a cascade involved in the regulation of several cellular processes, including organ size, seems to be an important candidate to as a mediator of ECM signals that change gene expression programs. Our laboratory has been exploring the hypothesis that Hippo is involved in crucial processes of mammary gland development and biology and it can intermediate the signals of the ECM that regulate these processes. Our preliminary results indicate that the tissue architecture and the basement membrane, an ECM component of epithelia and other tissues, influence the location, concentration and activity of YAP, an effector of the Hippo pathway. In this context, the goal of this proposal is to identify the proteins that interact with YAP in mammary epithelial cells in response to the basement membrane. We will use proximity-dependent biotin identification (BioID) followed by mass spectrometry analysis. We expect that the identification of YAP molecular partners will reveal an interaction network that may bring crucial information on the ECM-signaling mechanisms during mammary gland morphogenesis and homeostasis.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PEREIRA JÚNIOR, Antonio Carlos Manucci. Identification of interacting proteins of Yes-Associated Protein (Yap) in mammary epithelial cells exposed to laminin-rich extracellular matrix. 2019. Master's Dissertation - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.