Development of rapid microbiological methods (RMMs) for evaluation of potency of antimicrobial agents and their measurement uncertainties

• Grant number: 16/04100-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2016
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Felipe Rebello Lourenço
• Grantee: Alessandro Morais Saviano
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Agar diffusion method has been used in the evaluation of antimicrobial activity since the discovery of penicillin. Nevertheless, little progress has occurred in order to reduce the time required for the determination of growth inhibition zones. The goal of this project is to develop, optimize and validate rapid microbiological methods for evaluation of potency of antimicrobials, as well as to identify, quantify and assess the main sources ofuncertainty associated with potency. The project will be divided into four steps: step 1) determination of the parameters involved in the formation of inhibition zones and study of mechanisms of diffusion and microbial growth~ 2) influence of culture medium composition on inhibition zones~ 3) development, optimization and validation of rapid microbiological methods for the determination of antimicrobials potencies~ and 4) study of measurement uncertainty associated with antimicrobials potencies. We hope that the results of this project will allow the reduction of the time required for the determination of inhibition zone sizes. Additionally, we hope to contribute to the elucidation of the mechanisms of diffusion and microbial growth, making it possible to identify,quantify and improve the main sources of measurement uncertainty associated with formation of inhibition. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAVIANO, ALESSANDRO MORAIS; LOURENCO, FELIPE REBELLO. Measurement uncertainty estimation based on multiple regression analysis (MRA) and Monte Carlo (MC) simulations - Application to agar diffusion method. MEASUREMENT, v. 115, p. 269-278, FEB 2018. (16/04100-8)

SAVIANO, ALESSANDRO MORAIS; LOURENCO, FELIPE REBELLO. Rapid microbiological methods (RMMs) for evaluating the activity of cephalosporin antibiotics employing triphenyltetrazolium chloride. Talanta, v. 185, p. 520-527, AUG 1 2018. (16/04100-8)

SAVIANO, ALESSANDRO MORAIS; BETTENCOURT DA SILVA, RICARDO J. N.; LOURENCO, FELIPE REBELLO. Measurement uncertainty for the potency estimation by rapid microbiological methods (RMMs) with correlated data. REGULATORY TOXICOLOGY AND PHARMACOLOGY, v. 102, p. 117-124, MAR 2019. (16/04100-8)

SAVIANO, ALESSANDRO MORAIS; LOURENCO, FELIPE REBELLO. Using image analysis to determine gentamicin potency by agar diffusion microbiological assay and its measurement uncertainty. MEASUREMENT, v. 146, p. 315-321, NOV 2019. (16/04100-8, 17/04539-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SAVIANO, Alessandro Morais. Development of rapid microbiological methods (RMMs) for evaluation of potency of antimicrobial agents and their measurement uncertainties. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.