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Integrated analysis differential expression of miRNAs and mRNA associated with B chromosomes in Astatotilapia latifasciata cichlid.

Grant number: 16/09320-6
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2016
Effective date (End): February 28, 2018
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal Investigator:César Martins
Grantee:Jordana Inácio Nascimento Oliveira
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The supernumerary B chromosomes (Bs) are found in several eukaryotic organisms and do not follow mendelian segregation patterns. Bs has self transmission mechanism (drive) and this is poorly understood. B genomic constitution revels a mosaic of sequences including repetitive DNA, genes and peseudogenes. Studies have related this element to several functions like genetic variability into population, fertility effects, adaptation and evolutionary aspects to sexual chromosomes origin. These chromosomes are observed in some African cichlid's groups, being, sometimes, present in individuals of only one sex. In Astatotilapia latifasciata this chromosome is present both in males and females. However, few studies showed information about function and behavior of this element in the genome. Large scale data of the transcriptome and microRNome for this cichlid has revealed transcriptional differential activity within sex groups and, yet, B- and B+ individuals. Thus, perspectives for functional investigations show promissory to understand about B chromosome behavior. This study aim to understand the relation among mRNA and miRNA transcripts altered in B presence in A. latifasciata gonads tissue and the possible association between sex and B chromosome presence, though of differential transcription reported in mRNA and miRNA libraries.