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The role of mast cells and renin-angiotensin system on the inflamatory response induced by experimental periodontitis in mice

Grant number: 16/05780-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2016
Effective date (End): April 30, 2017
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Sandra Helena Penha de Oliveira
Grantee:Jessica Pinati Pereira
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated research grant:15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach, AP.TEM

Abstract

Periodontal disease (PD) is considered a chronic inflammatory condition and destructive of the supporting structures of the teeth and when developed by a diabetic patient manifests itself in an exaggerated way, promoting the release of inflammatory mediators. Induction of diabetes in animals has been an important tool to investigate the mysteries of this disease in the periodontal medicine. Mast cells (MCs) are cells found in both normal as inflamed periodontal tissue, thus it is assumed that the MCs can participate in a possible inflammatory response, however, the mechanisms by which these MCs may contribute to the progression of PD are still unknown .The renin-angiotensin system (RAS) is characterized as a regulator of blood pressure and long-term control of blood pressure. This SRA simply and clearly modulates several physiological functions such as blood pressure, and tissue inflammation. In diabetic patients with PD, treatment with inhibitor of angiotensin converting enzyme, induced an improvement in glucose levels, as well as the individual in periodontal conditions. However, it is not completely elucidated the mechanisms involved in this improvement. As long as the objective of this study is to evaluate the role of MCs and SARS on the inflammatory response of diabetic mice subjected to periodontitis. Initially will be used BALB/c mice to be euthanized 15 days after the ligation, and this time the regions close to the ligature, gum tissue, jaw and blood will be collected for laboratory processing for the study of inflammatory response. Arising from whole process will be conducted qualitative and quantitative analyzes (histology) of changes in the inflamed tissue through the analysis by optical microscopy of sections stained with H & E. The results, together, contribute to the improvement of knowledge of the molecular and cellular mechanisms involved in the inflammatory response, and can thereby improve the profile of the treatment of diabetic patients, contributing to the development of new therapies for the control of periodontitis the presence of diabetes .