Functional study of a disintegrin from Crotalus durissus collilineatus venom

• Grant number: 16/11578-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2016
• End date: July 31, 2017
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Eliane Candiani Arantes Braga
• Grantee: Rafaella Varzoni Manzini
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis, AP.TEM

Abstract

Snakebite envenomings are a problem to national public health because of its frequency and severity. Cases involving snakes of genus Crotalus are more severe, showing higher mortality rate. The venom of Crotalus durissus collilineatus is a complex mixture of several compounds, among them, the disintegrins. These proteins act as integrin inhibitor, a cell adhesion protein,, triggering translational responses that can lead the cells to apoptosis. The objectives of this project are the isolation and structural and functional characterization of a disintegrin from Crotalus durissus collilineatus venom. The snake venom will be fractionated on reversed-phase chromatography on a C-18 column and the fraction containing the disintegrin will be submitted to additional chromatographic processes to isolate the disintegrin. The structural characterization of the molecule will be performed by amino-terminal sequencing and molecular mass determination by mass spectrometry. The cell adhesion and cell migration (wound heaving) assays will be performed in HepG2 cell lines, aiming the functional characterization of the disintegrin. Due to the possible action of disintegrins in thrombosis, angiogenesis, metastases, inflammation and other diseases related to integrins, this project is of great relevance, since it seeks to unravel the action of disintegrin C. d. Collilineatus in cancer Due to the possible action of the disintegrins in thrombosis, angiogenesis, metastases, inflammation and others diseases related to integrins, this project is of great relevance, since aims to clarify the action of the disintegrin from C. d. collilineatus venom in cancer cells.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ISADORA SOUSA; MANZINI, RAFAELLA VARZONI; FERREIRA, ISABELA GOBBO; CARDOSO, IARA AIME; FIGUEIREDO BORDON, KARLA DE CASTRO; THOMAZELA MACHADO, ANA RITA; GREGGI ANTUNES, LUSANIA MARIA; ROSA, JOSE CESAR; ARANTES, ELIANE CANDIANI. Cell migration inhibition activity of a non-RGD disintegrin from Crotalus durissus collilineatus venom. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, OCT 20 2018. (16/11578-1, 11/23236-4, 12/14996-8, 17/03580-9)