Transcription factors are regulatory proteins that bind to specific DNA regions in response to various signals, controlling gene expression. The MarR family transcription factors act as direct sensors of multiple signals and control various processes in bacteria, such as virulence, antibiotic resistance and response to oxidative stress. Chromobacterium violaceum, a free-living beta-proteobacterium that can act as a human opportunistic pathogen, has fifteen MarR family transcription factors, some of which are being studied in our laboratory. This project aims to characterize the function and find out which genes are regulated by the MarR family transcription factor CV3905 in C. violaceum. To find genes regulated by CV3905, we will perform DNA microarray analysis with subsequent validation of selected genes. The CV3905 DNA binding sites will be defined by EMSA assays and in silico analysis. Based on the regulon found for CV3905, we will perform phenotypic testing, using a CV3905 mutant strain already available in the lab, to discover the function of this transcription factor. Thus, the data obtained in this project should help to understand the role of CV3905 in C. violaceum and, in a more broad sense, to increase our knowledge about MarR family transcription factors in bacteria.
News published in Agência FAPESP Newsletter about the scholarship: