Screening of Substrates and Inhibitors for epoxide hydrolase from Trichoderma reesei

• Grant number: 16/12859-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: November 01, 2016
• End date: April 20, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Felipe Santiago Chambergo Alcalde
• Grantee: Gabriel Stephani de Oliveira
• Supervisor: Bruce D. Hammock
• Host Institution: Escola de Artes, Ciências e Humanidades (EACH). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of California, Davis (UC Davis), United States
• Associated to the scholarship: 15/03329-9 - Cloning and characterization of the enzymes epoxide hydrolases from Trichoderma reesei, BP.DD

Abstract

Epoxide hydrolases (EHs) are enzymes that are present in all living organisms and catalyze the hydrolysis of epoxides to the corresponding vicinal diols. EHs have biotechnological potential in chiral chemistry and as a target for drug design. In the main Ph.D. Project (2015/03329-9), we have identified for the first time, an epoxide hydrolase enzyme from Trichoderma reesei fungus (TrEH) that was successfully cloned, expressed, purified and characterized as enantioselective, which is an important characteristic for chiral chemistry for industries. The aim of this Ph.D. internship abroad (BEPE-DD) at Hammock Laboratory in the University of California, Davis (UC-Davis, USA), is to identify substrates and inhibitors of TrEH, which facilitate the assessment of their roles in fungal metabolism. Understanding how this EH enzyme of model organism T. reesei responds to inhibitors may substantially contribute for the study of fungal metabolism and development of drugs for the pharmaceutical industry. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, GABRIEL S.; ADRIANI, PATRICIA P.; RIBEIRO, JOAO AUGUSTO; MORISSEAU, CHRISTOPHE; HAMMOCK, BRUCE D.; DIAS, MARCIO VINICIUS B.; CHAMBERGO, FELIPE S.. The molecular structure of an epoxide hydrolase from Trichoderma reesei in complex with urea or amide-based inhibitors. International Journal of Biological Macromolecules, v. 129, p. 653-658, MAY 15 2019. (13/15906-5, 16/12859-4, 15/03329-9, 15/09188-8, 18/00351-1, 14/24107-1, 17/25705-8)

DE OLIVEIRA, GABRIEL S.; ADRIANI, PATRICIA P.; WU, HAO; MORISSEAU, CHRISTOPHE; HAMMOCK, BRUCE D.; CHAMBERGO, FELIPE S.. Substrate and inhibitor selectivity, and biological activity of an epoxide hydrolase from Trichoderma reesei. MOLECULAR BIOLOGY REPORTS, v. 46, n. 1, p. 371-379, FEB 2019. (16/12859-4, 15/03329-9, 14/24107-1, 17/25705-8)