Cartilaginous and bone extracellular matrix: effect of collagen supplementation in animal model of osteoarthritis

• Grant number: 16/12367-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2016
• End date: July 31, 2018
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Fernando Augusto Vasilceac
• Grantee: Camila Marques de Araújo
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 13/00798-2 - The extracellular matrix in aging, exercise and in the tumor microenvironment, AP.TEM
• Associated scholarship(s): 17/03565-0 - Modeling pain in mice with experimental osteoarthritis, BE.EP.IC

Abstract

Previous studies report that the undenatured type II collagen has good results in the control of pain and improvement in physical function in osteoarthritis's patients. However, the remodeling of the cartilage extracellular matrix and the subchondral bone isn't clear in this context yet. Therefore, the aim of this study is to evaluate the cartilage extracellular matrix and the subchondral bone of knee osteoarthritis of rats after undenatured collagen supplementation. 24 rats will be divided into 4 groups: control (C), Osteoarthritis (OA), Control with supplementation (CS) and Osteoarthritis with supplementation (OS). The OA and OAS groups will be submitted to surgical transection of the anterior cruciate ligament (ACL) bilaterally, as an animal model of osteoarthritis. After 2 weeks of induction, the CS and OAS groups began the oral supplementation protocol, with 5mg of undenatured collagen diluted in 1 mL of water, daily for 2 weeks. At the end of the experiment, all groups will carry out the examination of bone densitometry to assess the subchondral bone. After euthanasia, all knee joints will be collected and processed for histological evaluation of the cartilage extracellular matrix according to the recommendations of OARSI (Osteoarthritis Research Society International). It has as perspective elucidating the intrinsic mechanism of collagen supplementation on knee osteoarthritis.