Therapeutic effect of administration of mesenchymal stem cells stimulated with collagen V in articular cartilage of rabbits with osteoarthritis

• Grant number: 14/11419-5
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): October 01, 2014
• Effective date (End): September 30, 2016
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Walcy Paganelli Rosolia Teodoro
• Grantee: Isabele Camargo Brindo da Cruz
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Osteoarthritis (OA) is a disease that affects the joints having as main characteristic the degeneration of articular cartilage. Currently, available treatments are ineffective in cartilage regeneration, often making it necessary surgery for placement of prostheses. In recent years many experimental and human studies have demonstrated the potential use of Mesenchymal Stem Cells (MSCs) in the treatment of OA. In this respect, MSCs derived from adipose tissue (ADSCs) have been a promising alternative due to their easy obtain, effective in bone and cartilage repair and stimulation of chondrogenesis by the Molecules of Extracelular Matrix (ECM). Among the ECM molecules, the Collagen V (Col V) due to its peculiar biochemical characteristics and importance in cell adhesion, migration, proliferation and differentiation, besides being present in large quantities in embryonic cartilage, could also stimulate chondrogenesis. Our proposal is to analyze whether the rabbits autologous ADSCs stimulated with Col V could have an effect on articular cartilage regeneration after induction of OA, restoring the characteristics of normal cartilage tissue and providing a direction for the development of future therapeutic protocols humans. Moreover, considering the easy obtaining adipose tissue and the difficulty of obtaining drugs that act on the osteoarthritic cartilage regeneration, verify if Col V stimulated human ADSCs can accelerate the chondrogenic process and therefore being useful in the treatment of OA. Furthermore, the results of this study will be very important to try clarifying the role of Col V in cultured ADSCs since this data can also be of great help in establishing new cellular models. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRINDO DA CRUZ, ISABELE CAMARGO; VELOSA, ANA PAULA PEREIRA; CARRASCO, SOLANGE; DOS SANTOS FILHO, ANTONIO; TOMAZ DE MIRANDA, JURANDIR; POMPEU, EDUARDO; FERNANDES, TIAGO LAZZARETTI; BUENO, DANIELA FRANCO; FANELLI, CAMILA; GOLDENSTEIN-SCHAINBERG, CLAUDIA; et al. Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 9, MAR 22 2021. (18/20403-6, 14/11419-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CRUZ, Isabele Camargo Brindo da. Therapeutic effect of the administration of mesenchymal stem cells stimulated with collagen V in the articular cartilage of rabbits with osteoarthritis. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.