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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage

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Brindo da Cruz, Isabele Camargo [1] ; Velosa, Ana Paula Pereira [1] ; Carrasco, Solange [1] ; dos Santos Filho, Antonio [1] ; Tomaz de Miranda, Jurandir [1] ; Pompeu, Eduardo [2] ; Fernandes, Tiago Lazzaretti [3, 4] ; Bueno, Daniela Franco [3] ; Fanelli, Camila [5] ; Goldenstein-Schainberg, Claudia [1] ; Fabro, Alexandre Todorovic [6, 7] ; Fuller, Ricardo [1] ; Silva, Pedro Leme [8, 9] ; Capelozzi, Vera Luiza [6] ; Teodoro, Walcy Rosolia [1]
Total Authors: 15
[1] Univ Sao Paulo, Hosp Clin, Fac Med, Rheumatol Div, FMUSP, Sao Paulo - Brazil
[2] Univ Sao Paulo, Hosp Clin, Fac Med, Bioterism Ctr, FMUSP, Sao Paulo - Brazil
[3] Hosp Sirio Libanes, Sao Paulo - Brazil
[4] Univ Sao Paulo, Hosp Clin, Fac Med, Sport Med Div, Inst Orthopaed & Traumatol, FMUSP, Sao Paulo - Brazil
[5] Univ Sao Paulo, Renal Div, Lab Cellular Genet & Mol Nephrol, Sao Paulo - Brazil
[6] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Pathol, FMUSP, Sao Paulo - Brazil
[7] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol & Legal Med, Resp Med Lab, Sao Paulo - Brazil
[8] Natl Inst Sci & Technol Regenerat Med, Rio De Janeiro - Brazil
[9] Univ Fed Rio de Janeiro, Carlos Chagas Filho Biophys Inst, Ctr Ciencias Saude, Lab Pulm Invest, Rio De Janeiro - Brazil
Total Affiliations: 9
Document type: Journal article
Web of Science Citations: 0

Collagen is essential for cartilage adhesion and formation. In the present study, histology, immunofluorescence, morphometry, and qRT-PCR suggested that adipose-derived stem cells (ADSCs) stimulated by type V collagen (Col V) induce a significant increase of type II collagen (Col II) in the degenerative area of surgical-induced osteoarthritic rabbit articular cartilage (OA). In vitro, the effects of Col V on the proliferation and differentiation of ADSC were investigated. The expression of the cartilage-related genes Col2a1 and Acan was significantly upregulated and Pou5fl was downregulated post-ADSC/Col V treatment. Post-ADSC/Col V treatment, in vivo analyses revealed that rabbits showed typical signs of osteoarthritic articular cartilage regeneration by hematoxylin and eosin (H\&E) and Safranin O/Fast Green staining. Immunohistochemical staining demonstrated that the volume of Col II fibers and the expression of Col II protein were significantly increased, and apoptosis Fas ligand positive significantly decreased post-ADSC/Col V treatment. In conclusion, the expression of Col II was higher in rabbits with surgical-induced osteoarthritic articular cartilage; hence, ADSC/Col V may be a promising therapeutic target for OA treatment. (AU)

FAPESP's process: 18/20403-6 - Biomolecular markers of proliferation and remodeling in acute and chronic respiratory diseases: promising therapeutic targets
Grantee:Vera Luiza Capelozzi
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/11419-5 - Therapeutic effect of administration of mesenchymal stem cells stimulated with collagen V in articular cartilage of rabbits with osteoarthritis
Grantee:Isabele Camargo Brindo da Cruz
Support Opportunities: Scholarships in Brazil - Master