| Grant number: | 16/15836-5 |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| Start date: | January 16, 2017 |
| End date: | January 15, 2018 |
| Field of knowledge: | Biological Sciences - Biophysics - Molecular Biophysics |
| Principal Investigator: | Wanius José Garcia da Silva |
| Grantee: | Viviam Moura da Silva |
| Supervisor: | Yvain Nicolet |
| Host Institution: | Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Santo André , SP, Brazil |
| Institution abroad: | Institut de Biologie Structurale (IBS), France |
| Associated to the scholarship: | 14/02065-5 - Modular Hyperthermostable endo-beta-1,4-mannanase: determination of the three-dimensional structure of the Central Domain an its role in the thermostabilization of whole enzyme, BP.DR |
Abstract The complete enzymatic hydrolysis of mannan polysaccharides requires at least three types of enzymes working synergistically: ²-mannanase, ²-mannosidase, and ²-glucosidase. The hyperthermophilic bacterium Thermotoga petrophila produces the enzyme endo-²-1,4-mannanse (TpMan), which is composed of a GH5 catalytic domain and a carbohydrate-binding domain connected through a linker. The crystal structure of the GH5 catalytic domain was determined, however, to date, there is no three-dimensional structure available for the full-length TpMan. Recently, we reported the first low-resolution model for the full-length TpMan and demonstrated that the linker is a compact domain. In this scientific project, as part of a continuing investigation of TpMan, we propose to determine the three-dimensional structure of the full-length enzyme (TpMan) and/or of its central domain (linker) using X-ray crystallography. Our previous attempts to crystallize the full-length TpMan and the linker were so far unsuccessful. Therefore, an internship in a center such as the Institut de Biologie Structurale - Grenoble/France with extensive experience in X-ray crystallography, should certainly be essential in determining the TpMan structure. (AU) | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
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