Role of ABA in water relations in metabolisms with contrasting sensitivities to Al3+

• Grant number: 15/25409-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2016
• End date: January 31, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Gustavo Habermann
• Grantee: Marina Alves Gavassi
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated scholarship(s): 18/08902-7 - ABA signaling for stomatal closure under Al stress, BE.EP.DR

Abstract

Aluminum (Al) is the most abundant element in the Earth's crust. In acidic soils (pH < 5.5) it is found as Al3+, which is toxic to most plants. In Al-sensitive plants, the first and most conspicuous symptom is the inhibition of the growth of the root system, where Al is covalently retained. Aluminum also causes indirect symptoms, such as reduced shoot growth. In Al-sensitive plants, Al-reduced CO2 assimilation rate (A) is observed, and there is evidence suggesting the impairment of photochemical reactions, as well as 30 to 80% reduction in stomatal conductance (gs). This suggests that when retained in the root system, the Al could affect the mesophyll hydration, like in a water deficit. On the other hand, plants from the Cerrado vegetation avoid Al uptake (Al non-accumulating species) and some species absorb Al but it does not cause any structural damage to their metabolism (Al-accumulating species). Based on these facts we will test the hypothesis that some of the symptoms of Al toxicity in sensitive plants, especially those related to the low growth and development of shoots, occur in response to the activation of mechanisms closely associated with the perception of water deficit, especially the biosynthesis of abscisic acid (ABA). We also expect that these associations do not occur in tolerant species, such as Styrax camporum. We will use S. camporum plants, which are considered moderate Al-accumulating species and 'Rangpur' lime plants (Citrus limonia), which are sensitive to Al. The two species will be studied separately, in nutrient solutions with and without Al. In these two groups of plants, in each experiment (S. camporum and C. limonia), we will measure the main root length, the shoot height, chlorophyll a fluorescence and gas exchange (including mainly gs). These non-destructive measurements will be made one day before the intervals of 1, 7, 15, 30, 45 and 60 days after planting (DAP). On these days, we will measure predawn and midday leaf water potentials, collect samples of roots and leaves for measuring the expression of the NCED gene, and the concentrations of ABA and its metabolites. After each collection, we will separate the organs (roots, stems + petioles, and leaves) to determine their respective dry masses. After that, these dried samples will be sent to a routine plant nutrition lab for quantification of the total Al contents in each of these organs.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GAVASSI, MARINA ALVES; SILVA, GISELLE SCHWAB; SANTIAGO DA SILVA, CAROLINA DE MARCHI; THOMPSON, ANDREW J.; MACLEOD, KYLE; RAYNER OLIVEIRA, PAULO MARCELO; CAVALHEIRO, MARIANA FEITOSA; DOMINGUES, DOUGLAS SILVA; HABERMANN, GUSTAVO. CED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stres. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 185, MAY 2021. (15/25409-4, 17/26144-0, 16/10896-0, 18/25658-2, 18/08902-7)

GAVASSI, MARINA ALVES; DODD, IAN CHARLES; PUERTOLAS, JAIME; SILVA, GISELLE SCHWAB; CARVALHO, ROGERIO FALLEIROS; HABERMANN, GUSTAVO. Aluminum-induced stomatal closure is related to low root hydraulic conductance and high ABA accumulation. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 179, NOV 2020. (15/25409-4, 18/08902-7)

SILVA, GISELLE SCHWAB; GAVASSI, MARINA ALVES; CARVALHO, BRENDA MISTRAL DE OLIVEIRA; HABERMANN, GUSTAVO. High abscisic acid and low root hydraulic conductivity may explain low leaf hydration in 'Mandarin' lime exposed to aluminum. TREE PHYSIOLOGY, v. N/A, p. 14-pg., 2022-11-09. (18/15359-8, 18/08902-7, 15/25409-4, 17/26144-0)

SILVA, G. S.; RODRIGUES, J. S.; CARVALHO, B. M. D. O.; GAVASSI, M. A.; BRESSAN, A. C. G.; HABERMANN, G.. Absence of aluminium compromises root integrity, reduces leaf hydration and Rubisco performance in Qualea grandiflora, an Al-accumulating species. Plant Biology, v. 25, n. 5, p. 10-pg., 2023-05-31. (18/15359-8, 18/08902-7, 17/26144-0, 15/25409-4)

GAVASSI, MARINA ALVES; ALVES, FREDERICO ROCHA RODRIGUES; MONTEIRO, CAROLINA CRISTINA; GAION, LUCAS APARECIDO; ALVES, LETICIA RODRIGUES; PRADO, RENATO DE MELLO; GRATA, PRISCILA LUPINO; CARVALHO, ROGERIO FALLEIOS. Photomorphogenic tomato mutants high-pigment 1 and aurea responses to iron. Scientia Horticulturae, v. 307, p. 9-pg., 2023-01-03. (13/04316-2, 15/25409-4, 18/08902-7)

GAVASSI, MARINA ALVES; SILVA, GISELLE SCHWAB; SANTIAGO DA SILVA, CAROLINA DE MARCHI; THOMPSON, ANDREW J.; MACLEOD, KYLE; RAYNER OLIVEIRA, PAULO MARCELO; CAVALHEIRO, MARIANA FEITOSA; DOMINGUES, DOUGLAS SILVA; HABERMANN, GUSTAVO. NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 185, p. 12-pg., 2021-02-06. (16/10896-0, 18/08902-7, 17/26144-0, 18/25658-2, 15/25409-4)

CAVALHEIRO, MARIANA F.; GAVASSI, MARINA A.; SILVA, GISELLE S.; NOGUEIRA, MATHEUS A.; SILVA, CAROLINA M. S.; DOMINGUES, DOUGLAS S.; HABERMANN, GUSTAVO. Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium. FUNCTIONAL PLANT BIOLOGY, v. 47, n. 2, p. 10-pg., 2020-01-01. (16/10896-0, 13/11370-3, 17/26144-0, 15/25409-4)

CAVALHEIRO, MARIANA F.; GAVASSI, MARINA A.; SILVA, GISELLE S.; NOGUEIRA, MATHEUS A.; SILVA, CAROLINA M. S.; DOMINGUES, DOUGLAS S.; HABERMANN, GUSTAVO. Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in `Rangpur' lime plants exposed to aluminium. FUNCTIONAL PLANT BIOLOGY, v. 47, n. 2, p. 112-121, 2020. (15/25409-4, 13/11370-3, 17/26144-0, 16/10896-0)

SILVA, GISELLE SCHWAB; GAVASSI, MARINA ALVES; NOGUEIRA, MATHEUS ARMELIN; HABERMANN, GUSTAVO. Aluminum prevents stomatal conductance from responding to vapor pressure deficit in Citrus limonia. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 155, p. 662-671, NOV 2018. (15/25409-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GAVASSI, Marina Alves. Role of ABA in water relations in species sensitive to Al3+. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.