New mechanisms for controlling triggered release of anticancer drugs in cellular backpacks

• Grant number: 16/10193-9
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: October 01, 2016
• End date: February 28, 2021
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Marisa Masumi Beppu
• Grantee: Rogério Aparecido Bataglioli
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 17/24668-1 - Responsive biosynthetic peptide sequences for use in self-assembled multilayered thin films, BE.EP.DD

Abstract

The majority of the chemotherapeutic drugs currently employed do not act exclusively into the target areas, which results in a series undesired effects to the patients. This scenario requires the design of strategies to overcome these drawbacks. The MIT research group lead by Prof. Michael F. Rubner and Prof. Robert E. Cohen has been developing through the layer-by-layer technique a new drug delivery device known as cell backpacks which might be attached to immune cell surface with no damage to cell viability. These backpacks present a top region for interaction with cells, an intermediate pay-load region and a bottom sacrificial region, responsible for detaching backpacks from substrate after production. Despite the recent scientific advances in both top and bottom cellular backpack regions, these device still requires meaningful developments into the pay-load region either in the drug loading and triggered release mechanisms. This research project proposes the study of the pay-load section of the cellular backpacks, aiming the development of new strategies for loading and triggered release of the anticancer drug doxorubicin into the backpacks. Besides the morphological and chemical characterization of the LbL films, this project also suggests testing different biocompatible materials as building-blocks of the pay-load region, in order to create a device able to release the drug as a response to external triggers such as magnetic field, near infrared radiation, among others. As the main project outcome the authors expect to develop the aforementioned strategies with proved efficacy in-vitro tests, which may support future in-vivo experiments with cellular backpacks. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BATAGLIOLI, R. A.; TAKETA, T. B.; ROCHA NETO, J. B. M.; LOPES, L. M.; COSTA, C. A. R.; BEPPU, M. M.. Analysis of pH and salt concentration on structural and model-drug delivery properties of polysaccharide-based multilayered films. Thin Solid Films, v. 685, p. 312-320, SEP 1 2019. (13/05135-1, 16/19976-6, 16/10193-9)

ROCHA NETO, J. B. M.; GOMES NETO, R. J.; BATAGLIOLI, R. A.; TAKETA, T. B.; PIMENTEL, S. B.; BARATTI, M. O.; COSTA, C. A. R.; CARVALHO, H. F.; BEPPU, M. M.. Engineering the surface of prostate tumor cells and hyaluronan/chitosan multilayer films to modulate cell-substrate adhesion properties. International Journal of Biological Macromolecules, v. 158, p. 197-207, SEP 1 2020. (16/19976-6, 16/10193-9, 13/05135-1)

MAIA ROCHA NETO, JOAO BATISTA; SOARES, ANDREY COATRINI; BATAGLIOLI, ROGERIO APARECIDO; CARR, OLIVIA; RODRIGUES COSTA, CARLOS ALBERTO; OLIVEIRA JR, OSVALDO N.; BEPPU, MARISA MASUMI; CARVALHO, HERNANDES F.. Polysaccharide Multilayer Films in Sensors for Detecting Prostate Tumor Cells Based on Hyaluronan-CD44 Interactions. CELLS, v. 9, n. 6, JUN 2020. (18/22214-6, 18/20560-4, 18/18953-8, 16/10193-9, 14/50938-8)

ROCHA NETO, J. B. M.; LIMA, G. G.; FIAMINGO, A.; GERMINIANI, L. G. L.; TAKETA, T. B.; BATAGLIOLI, R. A.; DA SILVEIRA, G. A. T.; DA SILVA, J. V. L.; CAMPANA-FILHO, S. P.; OLIVEIRA JR, O. N.; et al. Controlling antimicrobial activity and drug loading capacity of chitosan-based layer-by-layer films. International Journal of Biological Macromolecules, v. 172, p. 154-161, MAR 1 2021. (16/10193-9, 18/22214-6, 18/20560-4)

BATAGLIOLI, ROGERIO A.; LEAO, BRUNO S.; MILLS, CAROLYN E.; ROCHA NETO, JOAO BATISTA M.; TAKETA, THIAGO B.; OLSEN, BRADLEY D.; BEPPU, MARISA M.. Automated, High-Throughput Screening of Hybrid Elastin-like Polysaccharide/Polypeptide Multilayer Film Deposition. Biomacromolecules, v. N/A, p. 12-pg., 2023-06-22. (16/10193-9, 18/20560-4)

MAHL, CYNTHIA R. A.; TAKETA, THIAGO B.; BATAGLIOLI, ROGERIO A.; DE ARRUDA, EDUARDO JOSE; BEPPU, MARISA M.. Chitosan Functionalization with Amino Acids Yields to Higher Copper Ions Adsorption Capacity. JOURNAL OF POLYMERS AND THE ENVIRONMENT, v. 26, n. 12, p. 4338-4349, DEC 2018. (13/05135-1, 16/10193-9)

BATAGLIOLI, ROGERIO A.; ROCHA NETO, JOAO BATISTA M.; LEAO, BRUNO S.; GERMINIANI, LUIZ GUILHERME L.; TAKETA, THIAGO B.; BEPPU, MARISA M.. Interplay of the Assembly Conditions on Drug Transport Mechanisms in Polyelectrolyte Multilayer Films. Langmuir, v. 36, n. 42, p. 12532-12544, OCT 27 2020. (18/20560-4, 16/10193-9)

ROCHA NETO, J. B. M.; TAKETA, T. B.; BATAGLIOLI, R. A.; PIMENTEL, S. B.; SANTOS, D. M.; FIAMINGO, A.; COSTA, C. A. R.; CAMPANA-FILHO, S. P.; CARVALHO, H. F.; BEPPU, M. M.. Tailored chitosan/hyaluronan coatings for tumor cell adhesion: Effects of topography, charge density and surface composition. Applied Surface Science, v. 486, p. 508-518, AUG 30 2019. (16/19976-6, 13/05135-1, 16/10193-9)

CUNHA, BRUNO S.; BATAGLIOLI, ROGERIO A.; TAKETA, THIAGO B.; LOPES, LAISE M.; BEPPU, MARISA M.. Ionic liquid functionalization of chitosan beads for improving thermal stability and copper ions uptake from aqueous solution. JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING, v. 7, n. 3, JUN 2019. (16/10193-9, 13/05135-1)

MORRIS, MELODY A.; BATAGLIOLI, ROGERIO A.; MAI, DANIELLE J.; YANG, YUN JUNG; PALONI, JUSTIN M.; MILLS, CAROLYN E.; SCHMITZ, ZACHARY D.; DING, ERIKA A.; HUSKE, ALLISON C.; OLSEN, BRADLEY D.. Democratizing the rapid screening of protein expression for materials development. MOLECULAR SYSTEMS DESIGN & ENGINEERING, v. 8, n. 2, p. 13-pg., 2022-10-28. (16/10193-9, 17/24668-1)

TAKETA, THIAGO B.; BATAGLIOLI, ROGERIO A.; ROCHA NETO, JOAO BATISTA M.; BEPPU, MARISA M.. Probing axial metal distribution on biopolymer-based layer-by-layer films for antimicrobial use. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 199, MAR 2021. (16/10193-9, 18/20560-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BATAGLIOLI, Rogério Aparecido. Desenvolvimento de filmes finos à base de polímeros e polipeptídios recombinantes do tipo elastina a partir de métodos de triagem de alta produtividade. 2021. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química Campinas, SP.