Responsive biosynthetic peptide sequences for use in self-assembled multilayered thin films

• Grant number: 17/24668-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: March 21, 2018
• End date: March 20, 2019
• Field of knowledge: Engineering - Chemical Engineering - Chemical Technology
• Principal Investigator: Marisa Masumi Beppu
• Grantee: Rogério Aparecido Bataglioli
• Supervisor: Bradley Olsen
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: Massachusetts Institute of Technology (MIT), United States
• Associated to the scholarship: 16/10193-9 - New mechanisms for controlling triggered release of anticancer drugs in cellular backpacks, BP.DD

Abstract

Recent advances in molecular biology materials have enabled scientists to design, synthesize and conjugate various peptide sequences that encapsulate the functional characteristics of their natural protein systems. Despite their remarkable advantages in biocompatibility, biodegradability, therapeutic response, and low polydispersity (~1.0) over the traditional synthetic polymers, to our knowledge, few studies explored the use of these biosynthetic proteins in multilayered thin films prepared by the Layer-by-Layer (LbL) technique. This internship project aims the development of biosynthetic, smart-responsive, peptide sequences for future use in multilayered thin films for diagnostic and therapeutic use. The project will be conducted at Prof. Bradly D. Olsen's Lab, in the Chemical Engineering Department at MIT. At first, Prof. Olsen will train Rogerio in charged protein sequences C10 and charged variant sequences of Elastin-Like Polymers (EPLs) synthesis, both with well-established protocols in E. coli strains. Rogerio will work with Olsen to develop the machinery for automated protein screening, enabling him to develop and screen an extensive library of these charged proteins with different biofunctional ligands to promote cell binding and the degradation and release of materials, such as metalloproteases (MMP) cleavage site and the RGD integrin binding site. The researchers will screen and optimize the expression of these different genes in high throughput, allowing a large family of promising sequences to be identified during the one-year sandwich internship program. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORRIS, MELODY A.; BATAGLIOLI, ROGERIO A.; MAI, DANIELLE J.; YANG, YUN JUNG; PALONI, JUSTIN M.; MILLS, CAROLYN E.; SCHMITZ, ZACHARY D.; DING, ERIKA A.; HUSKE, ALLISON C.; OLSEN, BRADLEY D.. Democratizing the rapid screening of protein expression for materials development. MOLECULAR SYSTEMS DESIGN & ENGINEERING, v. 8, n. 2, p. 13-pg., 2022-10-28. (16/10193-9, 17/24668-1)