Investigation of the role of IRS1 in the beta-catenin signaling pathway in acute l...
| Grant number: | 16/17945-6 |
| Support type: | Scholarships abroad - Research Internship - Post-doctor |
| Effective date (Start): | November 30, 2016 |
| Effective date (End): | November 29, 2017 |
| Field of knowledge: | Biological Sciences - Biochemistry |
| Principal Investigator: | Hugo Aguirre Armelin |
| Grantee: | Matheus Henrique dos Santos Dias |
| Supervisor abroad: | Ian Prior |
| Home Institution: | Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil |
| Local de pesquisa : | University of Liverpool, England |
| Associated to the scholarship: | 12/20186-9 - Molecular mechanisms of functional interaction between Ras and cyclin D1 in Ras-driven malignant cells, BP.PD |
Abstract We propose that in Ras-driven malignant cells there is a functional interaction between cyclin D1 and this oncogene; in which the main function of cyclin D1 is to guarantee cell survival overcoming the Ras oncogenic stress. We showed in the presented reports that CRISPR-Cas9-mediated cyclin D1 depletion induces p53-independent senescence in Ras-driven carcinoma cells. Moreover, in MEFs cyclin D1 overexpression attenuates Ras-induced oncogenic stress enabling cell viability and proliferation. Elucidation of the molecular mechanisms underlying this putative functional interaction between Ras and cyclin D1 is the central goal of this project. These issues will be addressed by proteome and phosphoproteome network analyses followed by validation of key proteins involved in these molecular mechanisms. (AU) | |