Phylogeny of the Order Characiformes (Teleostei: Ostariophysi) using ultraconserved elements

• Grant number: 16/11313-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2016
• End date: November 30, 2020
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Claudio de Oliveira
• Grantee: Bruno Francelino de Melo
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 14/26508-3 - Phylogeny of the hyperdiverse order Characiformes (Teleostei: Ostariophysi) using ultraconserved elements, AP.TEM
• Associated scholarship(s): 18/24040-5 - Phylogenomics of the African characiform families Alestidae, Citharinidae, Distichodontidae and Hepsetidae, BE.EP.PD

Abstract

Order Characiformes, one of the world's largest components of the freshwater ichthyofauna with over 2,000 known species, is one of the least studied groups in terms of systematics. Members of this order always have great ecological and economic importance where they occur and are extensively used for human consumption or within the aquarium trade. A recent molecular study published by our research group proposed a new relationship hypothesis for Characiformes, recognized five new families and redefined Characidae. Although that study had provided a major advance in our knowledge on the systematics of Characiformes, there is still need for further phylogenetic studies, especially in relation to many highly species-rich groups within Characidae and several characiform families. A literature review reveals that there is also a necessity of using new and more resolutive markers since many of the interfamilial relationships are still poorly resolved. Thus, we are proposing a phylogenomic reconstruction of Characiformes employing modern techniques of next-generation sequencing using ultraconserved elements (UCEs), which have been shown as a powerful class of markers on the molecular systematics. Thus, the present study with a solid collaborative network of both labs from Brazil and USA, and will be fundamental for a better understanding of which monophyletic groups belong to Characiformes and to test hypotheses of interfamilial, and intergeneric relationships as well as the historical biogeography of Characiformes that is distributed throughout Ethiopic and Neotropical major hydrographic basins. (AU)

Scientific publications

(31)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MELO, BRUNO; SIDLAUSKAS, BRIAN; HOEKZEMA, KENDRA; VARI, RICHARD; DILLMAN, CASEY; OLIVEIRA, CLAUDIO. Molecular phylogenetics of Neotropical detritivorous fishes of the family Curimatidae (Teleostei: Characiformes). Molecular Phylogenetics and Evolution, v. 127, p. 800-812, OCT 2018. (16/11313-8, 14/26508-3, 11/08374-1, 13/16436-2)

MELO, BRUNO F.; SIDLAUSKAS, BRIAN L.; NEAR, THOMAS J.; ROXO, FABIO F.; GHEZELAYAGH, AVA; OCHOA, LUZ E.; STIASSNY, MELANIE L. J.; ARROYAVE, JAIRO; CHANG, JONATHAN; FAIRCLOTH, BRANT C.; et al. ccelerated Diversification Explains the Exceptional Species Richness of Tropical Characoid Fishe. Systematic Biology, v. 71, n. 1, p. 78-92, JAN 2022. (14/06853-8, 14/05051-5, 18/24040-5, 15/00691-9, 16/11313-8, 18/23883-9, 14/26508-3)

SIDLAUSKAS, BRIAN L.; ASSEGA, FERNANDO M.; MELO, BRUNO F.; OLIVEIRA, CLAUDIO; BIRINDELLI, JOSE L. O.. otal evidence phylogenetic analysis reveals polyphyly of Anostomoides and uncovers an unexpectedly ancient genus of Anostomidae fishes (Characiformes. ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 194, n. 2, p. 626-669, FEB 1 2022. (14/26508-3, 18/24040-5, 18/20610-1, 16/11313-8, 16/09204-6)

BRITSKI, HERALDO A.; MELO, BRUNO F.; VARI, RICHARD P.; OLIVEIRA, CLAUDIO. Revalidation and redescription of Steindachnerina nigrotaenia and redescription of S. insculpta (Characiformes: Curimatidae). Neotropical Ichthyology, v. 17, n. 1, 2019. (16/11313-8, 14/26508-3)

ROXO, FABIO F.; OCHOA, LUZ E.; SABAJ, MARK H.; LUJAN, NATHAN K.; COVAIN, RAPHAEL; SILVA, GABRIEL S. C.; MELO, BRUNO F.; ALBERT, JAMES S.; CHANG, JONATHAN; FORESTI, FAUSTO; et al. Phylogenomic reappraisal of the Neotropical catfish family Loricariidae (Teleostei: Siluriformes) using ultraconserved elements. Molecular Phylogenetics and Evolution, v. 135, p. 148-165, JUN 2019. (12/01622-2, 14/06853-8, 14/26508-3, 16/11313-8, 15/00691-9, 14/05051-5)

MATEUSSI, NADAYCA T. B.; MELO, BRUNO F.; FORESTI, FAUSTO; OLIVEIRA, CLAUDIO. Molecular Data Reveal Multiple Lineages in Piranhas of the Genus Pygocentrus (Teleostei, Characiformes). GENES, v. 10, n. 5, MAY 2019. (16/11313-8, 14/26508-3)

MELO, BRUNO F.; DORINI, BEATRIZ F.; FORESTI, FAUSTO; OLIVEIRA, CLAUDIO. Little Divergence Among Mitochondrial Lineages of Prochilodus (Teleostei, Characiformes). FRONTIERS IN GENETICS, v. 9, APR 4 2018. (16/11313-8, 14/26508-3, 11/08374-1, 13/16436-2)

MELO, BRUNO F.; ALBERT, JAMES S.; DAGOSTA, FERNANDO C. P.; TAGLIACOLLO, VICTOR A.. Biogeography of curimatid fishes reveals multiple lowland-upland river transitions and differential diversification in the Neotropics (Teleostei, Curimatidae). ECOLOGY AND EVOLUTION, v. 11, n. 22, NOV 2021. (18/20806-3, 16/19075-9, 11/08374-1, 16/11313-8, 14/26508-3, 17/09321-5)

BIRINDELLI, JOSE L. O.; MELO, BRUNO F.; RIBEIRO-SILVA, LUIS R.; DINIZ, DEBORA; OLIVEIRA, CLAUDIO. A New Species of Hypomasticus from Eastern Brazil Based on Morphological and Molecular Data (Characiformes, Anostomidae). Copeia, v. 108, n. 2, p. 416-425, JUL 2020. (16/11313-8, 14/26508-3)

BONANI MATEUSSI, NADAYCA T.; MELO, BRUNO F.; OLIVEIRA, CLAUDIO. Molecular delimitation and taxonomic revision of the wimple piranhaCatoprion(Characiformes: Serrasalmidae) with the description of a new species. Journal of Fish Biology, v. 97, n. 3, JUL 2020. (16/11313-8, 14/26508-3)

MELO, B. F.; OLIVEIRA, C.. Three new species of Curimatopsis (Characiformes: Curimatidae) from the Amazon basin. Journal of Fish Biology, v. 91, n. 2, p. 528-544, AUG 2017. (16/11313-8, 09/54931-0, 14/26508-3, 11/08374-1)

SERRANO, ERICA A.; MELO, BRUNO F.; FREITAS-SOUZA, DIOGO; OLIVEIRA, MARIA L. M.; UTSUNOMIA, RICARDO; OLIVEIRA, CLAUDIO; FORESTI, FAUSTO. Species delimitation in Neotropical fishes of the genus Characidium (Teleostei, Characiformes). ZOOLOGICA SCRIPTA, v. 48, n. 1, p. 69-80, JAN 2019. (16/11313-8, 14/26508-3, 13/02143-3)

BORTOLO, GUSTAVO C.; LIMA, FLAVIO C. T.; MELO, BRUNO F.. A New Cyphocharax from the Lower Rio Tapajos, Amazon Basin, Brazil (Characiformes: Curimatidae). Copeia, v. 106, n. 2, p. 346-352, JUL 2018. (16/11313-8, 16/04821-7, 13/20936-0, 11/51532-7, 14/26508-3)

ROXO, F. F.; SILVA, G. S. C.; MELO, B. F.. Hisonotus devidei, a new species from the SAo Francisco basin, Brazil (Siluriformes: Loricariidae). Journal of Fish Biology, v. 92, n. 6, p. 1687-1701, JUN 2018. (16/11313-8, 14/05051-5)

SANTOS, ROSIANE P.; MELO, BRUNO F.; YAZBECK, GABRIEL M.; OLIVEIRA, RAFAEL S.; HILARIO, HERON O.; PROSDOCIMI, FRANCISCO; CARVALHO, DANIEL C.. Diversification of Prochilodus in the eastern Brazilian Shield: Evidence from complete mitochondrial genomes (Teleostei, Prochilodontidae). JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, v. 59, n. 5, p. 1053-1063, JUL 2021. (16/11313-8)

MELO, BRUNO F.; SIDLAUSKAS, BRIAN L.; NEAR, THOMAS J.; ROXO, FABIO F.; GHEZELAYAGH, AVA; OCHOA, LUZ E.; STIASSNY, MELANIE L. J.; ARROYAVE, JAIRO; CHANG, JONATHAN; FAIRCLOTH, BRANT C.; et al. ccelerated Diversification Explains the Exceptional Species Richness of Tropical Characoid Fishe. Systematic Biology, v. 71, n. 1, p. 78-92, DEC 16 2021. (14/05051-5, 16/11313-8, 15/00691-9, 18/24040-5, 18/23883-9, 14/26508-3, 14/06853-8)

OCHOA, LUZ E.; DATOVO, ALESSIO; DONASCIMIENTO, CARLOS; ROXO, FABIO F.; SABAJ, MARK H.; CHANG, JONATHAN; MELO, BRUNO F.; SILVA, GABRIEL S. C.; FORESTI, FAUSTO; ALFARO, MICHAEL; et al. Phylogenomic analysis of trichomycterid catfishes (Teleostei: Siluriformes) inferred from ultraconserved elements. SCIENTIFIC REPORTS, v. 10, n. 1, FEB 14 2020. (15/13382-4, 14/06853-8, 14/26508-3, 16/11313-8, 15/00691-9, 16/19075-9, 14/05051-5)

FAIRCLOTH, BRANT C.; ALDA, FERNANDO; HOEKZEMA, KENDRA; BURNS, MICHAEL D.; OLIVEIRA, CLAUDIO; ALBERT, JAMES S.; MELO, BRUNO F.; OCHOA, LUZ E.; ROXO, FABIO F.; CHAKRABARTY, PROSANTA; et al. A Target Enrichment Bait Set for Studying Relationships among Ostariophysan Fishes. Copeia, v. 108, n. 1, p. 47-60, MAR 2020. (16/11313-8, 14/26508-3, 14/06853-8, 14/05051-5)

DORINI, BEATRIZ F.; RIBEIRO-SILVA, LUIS R.; FORESTI, FAUSTO; OLIVEIRA, CLAUDIO; MELO, BRUNO F.. Molecular phylogenetics provides a novel hypothesis of chromosome evolution in Neotropical fishes of the genus Potamorhina (Teleostei, Curimatidae). JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, v. 58, n. 4, JAN 2020. (18/24040-5, 16/11313-8, 14/26508-3)

MATEUSSI, NADAYCA T. B.; MELO, BRUNO F.; OTA, RAFAELA P.; ROXO, FABIO F.; OCHOA, LUZ E.; FORESTI, FAUSTO; OLIVEIRA, CLAUDIO. Phylogenomics of the Neotropical fish family Serrasalmidae with a novel intrafamilial classification (Teleostei: Characiformes). Molecular Phylogenetics and Evolution, v. 153, DEC 2020. (15/00691-9, 14/06853-8, 18/23883-9, 18/24040-5, 16/11313-8, 16/09204-6, 18/20610-1, 14/05051-5, 14/26508-3)

SOUZA, CAMILA S.; SILVA, GABRIEL S. C.; OCHOA, LUZ E.; ROXO, FABIO F.; COSTA-SILVA, GUILHERME J.; FORESTI, FAUSTO; MELO, BRUNO F.; OLIVEIRA, CLAUDIO. Molecular and morphological diversity in species of Kronichthys (Teleostei, Loricariidae) from Atlantic coastal rivers of Brazil. Journal of Fish Biology, v. 98, n. 3, DEC 2020. (18/23883-9, 17/06551-0, 14/26508-3, 16/11313-8)

SILVA, GABRIEL S. C.; MELO, BRUNO F.; ROXO, FABIO F.; OCHOA, LUZ E.; SHIBATTA, OSCAR A.; SABAJ, MARK H.; OLIVEIRA, CLAUDIO. hylogenomics of the bumblebee catfishes (Siluriformes: Pseudopimelodidae) using ultraconserved element. JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, v. 59, n. 8, JUL 2021. (16/11313-8, 15/00691-9, 14/26508-3, 16/19075-9)

MELO, BRUNO F.; TENCATT, LUIZ F. C.; OLIVEIRA, CLAUDIO. Phylogenetic Evidence for the Cyphocharax saladensis Clade with Description of a New Species of Cyphocharax Endemic to the Upper Rio Paraguai Basin (Teleostei: Curimatidae). ICHTHYOLOGY AND HERPETOLOGY, v. 110, n. 2, p. 13-pg., 2022-07-01. (14/26508-3, 16/11313-8)

DUTRA, G. M.; MELO, B. F.; NETTO-FERREIRA, A. L.. A new species of Curimatopsis Steindachner (Characiformes: Curimatidae) from the Rio Nhamunda, Amazon basin. Journal of Fish Biology, v. 92, n. 2, p. 515-522, FEB 2018. (16/11313-8, 11/50282-7, 09/54931-0)

BRUNA Q. URBANSKI; BRUNO F. MELO; GABRIEL S. C. SILVA; RICARDO C. BENINE. A new species of Tetragonopterus (Characiformes: Characidae) from Central Amazon lowlands, Brazil. Neotropical Ichthyology, v. 16, n. 2, 2018-06-11. (16/11313-8, 14/26508-3)

MELO, BRUNO F.. Cyphocharax boiadeiro, a new species from the upper rio Araguaia, Amazon basin, Brazil (Characiformes: Curimatidae). Zootaxa, v. 4247, n. 2, p. 114-120, MAR 27 2017. (16/11313-8, 09/54931-0, 14/26508-3, 11/08374-1)

BRUNO F. MELO. New species of Curimatopsis from the río Caroni, Orinoco basin, Venezuela, with comments on C. macrolepis (Characiformes: Curimatidae). Neotropical Ichthyology, v. 18, n. 2, 2020-06-17. (16/11313-8, 14/26508-3, 18/24040-5)

LUZ E. OCHOA; BRUNO F. MELO; JORGE E. GARCÍA-MELO; JAVIER A. MALDONADO-OCAMPO; CAMILA S. SOUZA; JUAN G. ALBORNOZ-GARZÓN; CRISTHIAN C. CONDE-SALDAÑA; FRANCISCO VILLA-NAVARRO; ARMANDO ORTEGA-LARA; CLAUDIO OLIVEIRA. Species delimitation reveals an underestimated diversity of Andean catfishes of the family Astroblepidae (Teleostei: Siluriformes). Neotropical Ichthyology, v. 18, n. 4, 2020-12-04. (18/09767-6, 17/06551-0, 16/19075-9, 16/11313-8, 18/23883-9, 14/26508-3, 14/06853-8)

SILVA, GABRIEL S. C.; ROXO, FABIO F.; MELO, BRUNO F.; OCHOA, LUZ E.; BOCKMANN, FLAVIO A.; SABAJ, MARK H.; JEREP, FERNANDO C.; FORESTI, FAUSTO; BENINE, RICARDO C.; OLIVEIRA, CLAUDIO. Evolutionary history of Heptapteridae catfishes using ultraconserved elements (Teleostei, Siluriformes). ZOOLOGICA SCRIPTA, v. 50, n. 5, p. 543-554, SEP 2021. (14/26508-3, 16/11313-8, 15/00691-9, 16/19075-9)

SOUZA, CAMILA S.; MELO, BRUNO F.; MATTOX, GEORGE M. T.; OLIVEIRA, CLAUDIO. Phylogenomic analysis of the Neotropical fish subfamily Characinae using ultraconserved elements (Teleostei: Characidae). Molecular Phylogenetics and Evolution, v. 171, p. 11-pg., 2022-04-04. (17/01970-4, 16/09204-6, 19/04602-1, 18/23883-9, 18/20610-1, 14/26508-3, 16/11313-8, 17/06551-0)

SOUZA, CAMILA S.; MATTOX, GEORGE M. T.; VITA, GEORGE; OCHOA, LUZ E.; MELO, BRUNO F.; OLIVEIRA, CLAUDIO. Molecular species delimitation and description of a new species of Phenacogaster (Teleostei, Characidae) from the southern Amazon basin. ZOOKEYS, v. N/A, n. 1164, p. 21-pg., 2023-05-26. (16/09204-6, 18/20610-1, 14/26508-3, 17/01970-4, 17/06551-0, 18/23883-9, 16/11313-8)