Thymus vulgaris L. (thyme) extract: analysis of antimicrobial activity on anaerobic bacteria and cytotoxicity in gingival fibroblasts

• Grant number: 16/17569-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2016
• End date: October 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Luciane Dias de Oliveira
• Grantee: Camila Cristina Panisello Ferreira
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Alternative methods for the control of pathogens have been employed every day more, as the application of plant products in materials intended for oral hygiene, so the evaluation of biocompatibility of these plant products also becomes relevant. Given this, the present study will be checked antimicrobial action of thyme extract on anaerobic species of dental interest, as Porphyromonas gingivalis (ATCC 33277) and Prevotella intermedia (ATCC 33563) in their planktonic and biofilm form, in addition to its effect on fibroblasts human gingival (FMM-1). Initially, minimum inhibitory (MIC) and bactericidal (MBC) concentrations will be performed by broth microdilution test. Subsequently, the most effective concentrations will be analyzed on biofilms of these species, with exposure of 5 min and 24 h. Chlorhexidine 0.12% and 0.9% saline solution will be used as positive and negative controls (n = 12 / group). The cytotoxicity of extract will be evaluate in gingival fibroblastos (FMN-1) by MTT, neutral red and crystal violet tests. Results are analyzed statistically by ANOVA and Tukey test (p d 0.05).