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Rosa centifolia L. glycolic extract: anti-inflammatory and antibiofilm activity on anaerobic bacteria of dental interest

Grant number: 19/16543-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2019
Effective date (End): August 31, 2020
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Luciane Dias de Oliveira
Grantee:Caroline Maria Ferreira
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

In recent years, there has been a significant increase about research in the world literature related to alternative therapies using natural products. These new substances of vegetable origin have shown promising results for biocompatibility. The objectives of this study are to analyze in vitro: a) antimicrobial and antibiofilm activity of R. centifolia L. glycolic extraction planktonic cultures and biofilms (monotypic) of Fusobacterium nucleatum e Porphyromonas gingivalis with differents contact times; b) cytotoxicity in macrophages (RAW 264.7); and c) anti-inflammatory action of R. centifolia L. extract in macrophages (RAW 264.7) stimulated by lipopolysaccharide (LPS) of Escherichia coli. For verification of antimicrobial activity of the extract, the microdilution broth method will be use, according to the Clinical and Laboratory Standards Institute (CLSI) M11-A7 on anaerobic microorganisms. The most effective concentration will be analyzed on biofilms for 5 min and 24 h. Subsequently, the cytotoxicity of the extract for RAW 264.7 will be evaluated with MTT test. The anti-inflammatory action of the extract will be verified by ELISA, with quantification of pro-inflammatory cytokines (IL-1² and TNF-±), after exposure of RAW 264.7 to the most effective concentrations of the extract, in the absence and presence of LPS. The results will be statistically analyzed by ANOVA and Tukey Test (p d 0.05).