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Evaluation of senescence involved in tissue injury induced by t. nattereri venom

Grant number: 16/15157-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2016
Effective date (End): October 31, 2017
Field of knowledge:Biological Sciences - Immunology
Principal Investigator:Carla Lima da Silva
Grantee:Fabiana Franco Carvalho
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Venomous fish are responsible for numerous accidents involving humans in the north and northeast regions of Brazil, Thalassophryne nattereri being the most common of them. T. nattereri venom causes chronic inflammation, impaired tissue repair and muscle necrosis in poisoned victims. In the inflammatory process, some cells such as neutrophils, monocytes and macrophages are recruited by inflammatory mediators for control and repair of the injury, but studies developed previously by our group show that the venom of T. nattereri develops an inappropriate cellular inflammatory response characterized by a reduced number of neutrophils at the site of injury. Infiltration of neutrophils and their subsequent elimination by macrophages after apoptosis is the prerequisite for the resolution of inflammatory processes, and knowing that the T. nattereri venom and its main toxin Natterins alter the normal process of leukocyte recruitment into the tissue allowing the persistence the lesion becomes necessary to extend our studies of the cellular events responsible for the transition from acute inflammatory response without resolution for a persistent, chronic and pathological settings. To gain new insights into the mechanisms of venom pathogenesis this work aims to investigate the profile of cellular response mainly phagocytic and antigen-presenting cells (macrophages and dendritic cells) on the inflamed tissue and in draining lymphoid organs, and evaluate the induction of senescent microenvironment in tissue lesion.