Characterizing stress response of DNA replication in the protozoan Leishmania: the case of replication-transcription conflict

• Grant number: 16/18191-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2016
• End date: October 31, 2019
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Agreement: BBSRC, UKRI
• Principal Investigator: Luiz Ricardo Orsini Tosi
• Grantee: Stela Virgilio
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites?, AP.TEM

Abstract

In this project we propose the identification of sites of conflict between the processes of replication and transcription in trypanosomatids using a non-targeted approach. We will use chromatin immunoprecipitation of proteins that bind to DNA structures related to replication stress and/or resolution of the replication-transcription clash. The use of high-throughput sequencing will allow the mapping of the identified sites. We will also evaluate the effect of replicative stress on the pattern of replication origin firing using the MFAseq approach. The findings will considerably advance our knowledge of the trypanosomatid biology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VELASQUEZ, ANGELA M. A.; BARTLETT, PAULA J.; LINARES, IRWIN A. P.; PASSALACQUA, THAIS G.; TEODORO, DAPHNE D. L.; IMAMURA, KELY B.; VIRGILIO, STELA; TOSI, LUIZ R. O.; LEITE, ALINE DE LIMA; BUZALAF, MARILIA A. R.; et al. ew Insights into the Mechanism of Action of the Cyclopalladated Complex (CP2) in Leishmania: Calcium Dysregulation, Mitochondrial Dysfunction, and Cell Deat. Antimicrobial Agents and Chemotherapy, v. 66, n. 1, JAN 2022. (16/18191-5, 16/05345-4, 18/23015-7, 20/04415-4, 17/03552-5, 16/19289-9, 19/21661-1)