Interaction of human immunoglobulin g onto epoxy chitosan/alginate adsorbents study: evaluation of adsorption isotherms by artificial neural networks

• Grant number: 16/18005-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2016
• End date: October 31, 2017
• Field of knowledge: Engineering - Chemical Engineering - Industrial Operations and Equipment for Chemical Engineering
• Principal Investigator: Igor Tadeu Lazzarotto Bresolin
• Grantee: Ana Carolina Moreno Pássaro
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Human immunoglobulin G (IgG) in an antibody used for several therapeutic indications. As high concentration and purity are required, selective adsorption techniques are recommended for recovery and purification purposes. Thus, this undergraduate research project aims to evaluate human IgG adsorption onto non-conventional adsorbents synthetized of chitosan and alginate with and without immobilized dyes as ligands (Reactive Green, Reactive Blue and Cibacron Blue). The adsorption will be evaluated under different experimental conditions, by varying the pH between 6.0 and 8.5 and the temperature between 4 and 37o C. The adsorption data modelling will be performed by using artificial neural networks and compared to classic non-linear methods for Langmuir, Langmuir-Freundlich and Temkin parameters adjusting. Thus, it will possible to obtain the necessary data to define the optimum adsorption condition aiming to improve the use of this technique in downstream processing of human IgG. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORENO PASSARO, ANA CAROLINA; MOZETIC, TAINA MAIA; SCHMITZ, JONES ERNI; DA SILVA JR, IVANILDO JOSE; MARTINS, TIAGO DIAS; LAZZAROTTO BRESOLIN, IGOR TADEU. Human Immunoglobulin G Adsorption in Epoxy Chitosan/Alginate Adsorbents: Evaluation of Isotherms by Artificial Neural Networks. CHEMICAL PRODUCT AND PROCESS MODELING, v. 14, n. 4, DEC 2019. (16/18005-7, 14/23893-3)