Comparison between two techniques for heart failure induction in rats: permanent coronary artery ligation and ischaemia-reperfusion

• Grant number: 16/19089-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): January 01, 2017
• Effective date (End): December 31, 2017
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Helio Cesar Salgado
• Grantee: Hadder Batista Silva
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 13/20549-7 - New insights in cardiovascular regulation under physiological and pathophysiological condition, AP.TEM

Abstract

One of the most important causes of heart failure (HF) is the ischemic heart disease. Thus, studies of experimental models of myocardial infarction (MI) are highly relevant for the pathophysiology of HF. MI induced by ligation of the left anterior descending branch of the left coronary artery (LAD), in experimental animals, leads to HF; however, this maneuver shows high mortality rates in rats. Myocardial reperfusion after coronary artery ligation can be performed in an attempt to recover the ischemic myocardium, but also can lead to the death of cardiomyocytes, and contribute to HF progression. Therefore, the ischemia-reperfusion technique (IR) will be examined for obtaining an experimental model of HF with lower mortality, as compared to permanent ligation of the LAD. The aim of this study in rats is the comparison of these two models, by assessing morphological and functional parameters of the heart. To accomplish this, thirty Wistar male rats will be distributed into three groups: 1) MI: permanent coronary artery ligation; 2) IR: 30 minutes ischemia followed by reperfusion; 3) SHAM: fictitious IR. After four weeks postoperatively, the animals will be evaluated by echocardiography, direct measurement of left ventricular pressure and plasma NT-proBNP dosage. (AU)