Phenotypic and genotypic characterization of aminoglycoside resistance in Acinetobacter baumannii

• Grant number: 16/20727-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): January 01, 2017
• Effective date (End): December 31, 2017
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Carlos Henrique Camargo
• Grantee: Daniel Almeida Sant'Ana
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Acinetobacter baumannii is one of the main bacterial agents associated with healthcare infections, mainly in immunocompromised patients. This species present intrinsic and acquired antimicrobial resistance determinants, reducing the therapeutic option for the treatment of infections. Over the last decades, carbapenems were one of the main antimicrobial agents used for the treatment of Acinetobacter infections, however, emergence and dissemination of carbapenemase enzymes led to the utilization of another classes of antimicrobial agents, such as the aminoglycosides. Aminoglycosides act by binding to specific ribosomal subunits, inhibiting the protein synthesis. Nevertheless, production of aminoglycoside modifying enzymes (AMEs) can inactivate these antimicrobials, leading to resistance. Thus, the aim of this study is to evaluate the antimicrobial susceptibility to aminoglycosides and to detect the main aminoglycoside modifying enzymes genes in a collection of Acinetobacter baumannii. A total of 40 non-repetitive A. baumannii strains were recovered from bloodstream hospitalized patients, identified by Vitek II, and stored for this study. Susceptibility to amikacin, gentamicin and tobramycin will be assessed by E-test, and the minimal inhibitory values will be classified in susceptible, intermediate and resistance according to CLSI 2016. The aac(3)-Ia, aac(3)-IIa, aac(62)-Ih, aph(32)-VI, ant(2")-Ia, aph(32)-Ia, aac(62)-Ib genes will be detected by multiplexed polymerase chain reaction. Correlation between the susceptibility profiles and the PCR results will be conducted. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRISOLLA TAVARES, LAIS CALISSI; VIEIRA CUNHA, MARCOS PAULO; DE VASCONCELLOS, FRANCIELLI MAHNIC; DE JESUS BERTANI, AMANDA MARIA; FRANCO DE BARCELLOS, THAYS ALMEIDA; BUENO, MARIANA SARDINHA; SANTOS, CARLA ADRIANA; SANT'ANA, DANIEL ALMEIDA; FERREIRA, ADRIANO MARTISON; MONDELLI, ALESSANDRO LIA; et al. Genomic and Clinical Characterization of IMP-1-Producing Multidrug-Resistant Acinetobacter bereziniae Isolates from Bloodstream Infections in a Brazilian Tertiary Hospital. MICROBIAL DRUG RESISTANCE, v. 26, n. 11, MAR 2020. (16/20727-0, 15/13179-4, 17/16988-6)

BRISOLLA TAVARES, LAIS CALISSI; DE VASCONCELLOS, FRANCIELLI MAHNIC; SANT'ANA, DANIEL ALMEIDA; TIBA-CASAS, MONIQUE RIBEIRO; CAMARGO, CARLOS HENRIQUE. Acinetobacter baumannii ST317 can be identified with Martins' trilocus sequence-based multiplex-PCR. INFECTION GENETICS AND EVOLUTION, v. 58, p. 251-252, MAR 2018. (16/20727-0, 15/13179-4)