Low-level laser therapy action on histological and muscle strength aspects at long term after skeletal muscle trauma in diabetic rats

• Grant number: 16/20058-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2017
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Rodrigo Leal de Paiva Carvalho
• Grantee: Ana Alice Teixeira Napolitano
• Host Institution: Centro de Ciências da Saúde. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil

Abstract

Diabetes mellitus (DM) is a heterogeneous group of metabolic disorders that have in common hyperglycemia, characterized by inadequate insulin secretory response, defects in insulin action, or both. Muscle injuries often take a long time treatment. Muscular regeneration is a complex process that leads to morphological changes of the regenerated fibers. It is known that skeletal muscle repair process in rats lasts about 28 days after the injury, however little is known about the histological recovery and muscle strength at the end of the muscle tissue repair process in a diabetic organism. Different types of therapies, pharmacological and non-pharmacological, have been used in the treatment and recovery of musculoskeletal injuries, such as anti-inflammatory drugs and low level laser therapy. The aim of this study is to evaluate the recovery of muscle tissue during and after the repair process (28 days), by histological recovery and muscle strength analysis after muscle injury trauma in diabetic rats, using as treatment low level laser therapy and diclofenac, alone or in combination. For injury induction the animals receive a single trauma in the right hind limb. The laser irradiation will be performed at only one point in the trauma area, at 3J energy dose for 30s. Diclofenac will be applied one hour after injury protocol, at a dose of 11.6 mg/g -1 (milligram gram) topically, locally. Histological recovery and muscle strength analysis of the muscle tissue will be performed at 28 days after injury induction.