Biomechanical jejunum analysis of dogs' corpses submitted to up to 4 months formaldehyde fixation and conservation in 30% sodium chloride aqueous solution

• Grant number: 16/00438-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2016
• End date: November 30, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Fabrício Singaretti de Oliveira
• Grantee: Isabella Cristina de Castro Lippi
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

In order to avoid tissue deterioration, anatomical pieces are fixed. Formaldehyde is the most used fixing material and preservative, and, although it is still very useful at anatomy labs, it's hazardous and also brings lots of health risks. Other agents, as the Sodium chloride, have been tested as preservative for anatomical pieces. Besides, it is getting more and more important to have a lot of criteria about animal utilization in research and teaching activities and, consequently, look out for alternative methods for these uses, in a way that will not cause scientific or academic losses. The aim of this study is to check the biomechanical effect inflicted on the jejunum of corpses of dogs that were submitted to up to 4-month formaldehyde fixation and conservation on a 30% sodium chloride aqueous solution. Changes on intestine resistance will be analyzed, measured in Newtons or Kgf, during the biomechanical traction test, on the moments 30, 60, 90 and 120 days of fixation on formaldehyde and 30, 60, 90 and 120 days of conservation on a 30% aqueous solution sodium chloride. It will be achieved if the 4-month-time fixation, with posterior conservation on aqueous solution sodium chloride, changes the intestine resistance. The comparison with the control group (fresh corpses) will define if there is data similarity, recommending or not those corpses for surgical technique teaching in intestine tissue. (AU)