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Microscopy as a tool for cellular alterations evaluation of leaf-cutting ants exposed to non-specific agrochemicals: analysis at ultrastructural level and confocal microscope

Grant number: 16/18916-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): December 01, 2016
Effective date (End): November 30, 2019
Field of knowledge:Biological Sciences - Ecology - Applied Ecology
Principal Investigator:Odair Correa Bueno
Grantee:Silvana Beani Poiani
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated research grant:12/25299-6 - Integrated studies for leaf cutting control, AP.TEM
Associated scholarship(s):17/19718-0 - The influence of xenobiotics on antioxidant enzymes of fat body of Atta sexdens rubropilosa workers (Myrmicinae, Attini), BE.EP.PD


This project aims to evaluate the effects of pesticides, as well as other environmental contaminants used in the cultivation of sugarcane in the cell death of leaf cutter ant, Atta sexdens rubropilos, by analyzing the viability of brain cells, digestive tract and Malpighian tubules, as well as the evaluation of the possible changes that may occur in the structure of the cytoskeleton and the nucleus of these cells. For this, it will be applied the technique of confocal microscopy, in which markers of subcellular structures will be used. (AU)