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Effects of laser biomineralization with dicalcium and tetracalcium phosphate-based on permeability and bond strength in human dentin

Grant number: 16/20437-2
Support Opportunities:Scholarships abroad - Research
Effective date (Start): March 15, 2017
Effective date (End): March 14, 2018
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:César Rogerio Pucci
Grantee:César Rogerio Pucci
Host Investigator: Franklin Chi-Meng Tay
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Research place: Augusta University, United States  


The objective of this study is to evaluate in vitro the effect of the use of dIcalcium and tetracalcium phosphate-base and application of Nd:YAG laser in the permeability and bond strength of human dentin. The application technique of the universal adhesive system and Nd:YAG laser will modifying for the purpose to elaborate a dentine with permeability reduced. Dentine discs will be used withapproximately 6mm in diameter and 1,5 mm height, obtained from healthy human molars. Dentinpermeability is measured in four stages: initial, maximum, during treatment and after treatment. After specimens standardization(n = 40) will be divided into two groups with (CFC) and without (SFC) dicalcium and tertacalcium phosphate application and then will be again divided into two subgroups,resulting in 4 subgroups (n = 10): AL- Adhesive + Laser; FAL- Calcium phosphate + Adhesive + Laser;4 LAL- Laser + adhesive + Laser; FLAL- Calcium phosphate + Laser + Adhesive + Laser. The application of Nd: YAG laser with energy of 60 mJ will be without contact, and after treatments the specimens will evaluate in FT-IR. The Single Bond Universal (3M-ESPE) will be used and after curing will be restored with Filtek Z 350 XT (3M ESPE) of approximately 3mm thickness. The specimens will be submitted to 10000 thermal cycles, and later will be used microtensile test. Data from both evaluations will be submitted to ANOVA and Tukey's test (± = 5%). In addition they will be observed inscanning electron microscopy (SEM) and Transmission electron microscopy (TEM). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BENITEZ SELLAN, PABLO LENIN; ZANATTA, RAYSSA FERREIRA; GOMES TORRES, CARLOS ROCHA; TIAN, FU-CONG; BERGERON, BRIAN E.; NIU, LI-NA; PUCCI, CESAR ROGERIO. Effects of calcium-phosphate, laser and adhesive on dentin permeability and bond strength. HELIYON, v. 6, n. 5, . (16/20437-2)
PUCCI, CESAR R.; GU, LI-SHA; ZHANG, HONG-YUN; SONG, QUN; XIA, VERONICA WANG; DAVIS, LINSEY B.; ANDRADE, DANILO DE SOUZA; MAZZONI, ANNALISA; BRESCHI, LORENZO; PASHLEY, DAVID H.; et al. Water-associated attributes in the contemporary dentin bonding milieu. Journal of Dentistry, v. 74, p. 79-89, . (16/20437-2)

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