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Endothelial barrier disruption in immunoprivileged organs during spontaneous canine visceral leishmaniasis: brain and eyeball endothelial junctional protein study

Grant number: 16/23255-2
Support type:Scholarships abroad - Research
Effective date (Start): February 03, 2017
Effective date (End): April 02, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Gisele Fabrino Machado
Grantee:Gisele Fabrino Machado
Host: Ingeborg Maria Langohr
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Local de pesquisa : Louisiana State University (LSU), United States  

Abstract

Visceral leishmaniasis is a complex disease caused by Leishmania donovani and Leishmania infantum with worldwide distribution. In dogs, besides the classical symptoms of the disease, there are descriptions of inflammatory alterations in the brain, neurological symptoms and in some rare cases, the presence of the parasite. The detection of antibodies in the anterior chamber of animal eyes could be related to the uveitis processes in the infected animal. Both, brain and eyeball inflammation is a strict controlled process and counts on the efficiency of the blood barriers that are paramount for the immune privilege of those organs. With the present study we aimed to assess endothelial junctional proteins of brain and eyeballs of dogs with spontaneous visceral leishmaniasis. To this end, we will use immunohistochemistry to evaluate endothelial junctional proteins (occludin and 5-claudin-5) stain patterns in the blood brain barrier, CSF-blood barrier, blood-aqueous barrier and in the blood-retinal barrier. Albumin and Ig-G levels in the cerebrospinal fluid (CSF) and aqueous humor will be quantified to access barriers function and we will use immunohistochemistry for detection of any perivascular leakage of albumin and IgG. DNA of Leishmania infantum will be searched on samples of brain tissue and in eyeballs using in situ hybridization.