Toxicogenic study of licochalcone a and its influence on genomic instability

• Grant number: 16/22863-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2017
• Effective date (End): December 31, 2017
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Denise Crispim Tavares Barbosa
• Grantee: Iara Silva Squarisi
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Licorice root has been used in traditional medicine in the East and West for the treatment of gastric ulcer, bronchial asthma and inflammation. Licochalcone A (LCA) is an important chalcone isolated from the root of Chinese licorice, Glycyrrhiza inflata Batalin. The literature reports that LCA has antitumor, anti-angiogenic, antiparasitic, antioxidant, antibacterial, anti-inflammatory and osteogenic activities. In view of the various biological activities, this study aims to evaluate the cytotoxic and genotoxic potential of LCA, as well as its effect on DNA damage induced by mutagens with different mechanisms of action, being these doxorubicin, hydrogen peroxide and etoposide. Cytotoxicity will be assessed by XTT colorimetric assay on Chinese hamster ovary fibroblasts (CHO cells). For the evaluation of the genotoxic potential of LCA and its effect on the genotoxicity induced by different mutagens will be used the micronucleus test in CHO cells. As a parameter of analysis, the frequency of micronuclei in 3000 binucleated cells per treatment group will be analyzed. The cytotoxicity of the treatments will also be assessed by the nuclear division index, where 1500 cells per treatment will be analyzed. In this way, the study in question will allow a better understanding of the mechanisms of LCA action. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE FREITAS, KAROLINE SOARES; SQUARISI, IARA SILVA; ACESIO, NATHALIA OLIVEIRA; NICOLELLA, HELOIZA DINIZ; OZELIN, SAULO DUARTE; REIS SANTOS DE MELO, MATHEUS; GUISSONE, ANA PAULA PRADO; FERNANDES, GABRIELA; SILVA, LIVIA MARA; DA SILVA FILHO, ADEMAR ALVES; et al. Licochalcone A, a licorice flavonoid: antioxidant, cytotoxic, genotoxic, and chemopreventive potential. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 83, n. 21-22, SEP 2020. (17/06070-1, 16/22863-9)