| Grant number: | 16/19775-0 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | March 01, 2017 |
| End date: | October 13, 2019 |
| Field of knowledge: | Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Anete Pereira de Souza |
| Grantee: | Clelton Aparecido dos Santos |
| Host Institution: | Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Associated scholarship(s): | 17/17390-7 - Substrate Specificity, Unusual Enzymatic Activity, and Synergistic Relationships of beta-glucosidases from Trichoderma harzianum, BE.EP.PD |
Abstract The conversion of sugar derived from plant biomass degradation via enzymatic hydrolysis for biofuels production is a challenge. Therefore, the search for new microorganisms and enzymes that can increase the efficiency of the saccharification of cellulosic substrates remains an important and high-priority area of study. Using a rational approach, comparing RNA-Seq and structural genomics data, our group has been able to identify the major beta-glucosidase expressed by Trichoderma harzianum (ThBgl) under biomass degradation conditions. However, after the structural and functional ThBgl characterization, the results showed that although this enzyme has been produced in high yield and purity, it did not have the two major properties desired for its effective use to supplement enzyme cocktails to the degradation of biomass - thermostability, and highglucose tolerance. In this context, the goals of this project are to provide new insights into protein engineering field, especially those related in the degradation of plant biomass, with the implementation of a platform using site-directed mutagenesis (SDM) and chimeric proteins design for the development of more efficient enzymes to be used in the supplemental enzyme cocktails to the saccharification of cellulosic substrates. (AU) | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
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