| Grant number: | 16/25735-1 |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate (Direct) |
| Start date: | April 01, 2017 |
| End date: | March 31, 2018 |
| Field of knowledge: | Physical Sciences and Mathematics - Chemistry - Organic Chemistry |
| Principal Investigator: | Luciana Gonzaga de Oliveira |
| Grantee: | Renata Sigrist |
| Supervisor: | Bradley S. Moore |
| Host Institution: | Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Institution abroad: | University of California, San Diego (UC San Diego), United States |
| Associated to the scholarship: | 15/01013-4 - Mining a t1PKS-transAT PKS-NRPS in Streptomyces sp. genome, BP.DD |
Abstract Actinobacteria are a phylum of gram-positive terrestrial or aquatic bacteria of significant importance for pharmaceutical industry and academic research due to their great potential to biosynthesize different types of natural products, including antibiotics and anticancer agents. Streptomyces is the largest bacterial genera from this phylum and several sequenced genomes have revealed an abundance of gene clusters codifying for enzymes from secondary metabolism demonstrating an underestimated potential to produce important bioactive metabolites. Among the most useful natural products, those biosynthesized by nonribosomal peptides synthetases and polyketide synthases share both high molecular complexity and therapeutic activity. A hybrid NRPS - type I PKS gene cluster encoding the biosynthesis of a potential unknown metabolite was annotated from the whole genome sequencing of the endophytic strain Streptomyces sp. B1 isolated from Citrus ssp. In this internship project, complementing the current PhD project (2015/01013-4), we propose to perform transcriptional analysis by real-time quantitative PCR (qPCR) of the biosynthetic gene cluster in order to decipher the metabolite produced by the action of t1PKS-transATPKS-NRPS. Additionally, as a complementary approach of the recombination methodologies already explored we intend to apply transformation-associated recombinantion (TAR) direct cloning to promote the heterologous expression of an entire gene cluster in S. coelicolor host. (AU) | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
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