Modulator effect of ionizing radiation on cytokines blood and tissue cells bone present at interface bone-implant

• Grant number: 16/19707-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2017
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Luana Marotta Reis de Vasconcellos
• Grantee: Mariana Raquel da Cruz Vegian
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Dental implants are considered as effective rehabilitation treatment. However, the success of osseointegration is associated with the health of the peri-implant tissue. Several factors may adversely affect the receptor site, including, radiation therapy, what is the procedure in which occurs the release of radiation in specific areas, aiming the treatment of tumors. Radiotherapy has been associated with increased loss risk of implants and higher failure rate. However, the mechanisms by which these events take place have not yet been completely understood. O objective of this study is to evaluate the modulatory effect of radiotherapy, simulating total dose of a conventional radiation treatment for cancer patients, in expression of blood cytokines and in bone tissue cells. It will be used in this study 72 mice that receive threaded titanium implants commercially pure grade 2 (cpTi) the right femurs. The animals will be divided in 4 groups: a) implant group (Ip): placement of the implant surgery (Group control - healthy tissue without irradiation); b) Group early irradiation (IRP): surgery implant placement followed by irradiation after 1 day; c) Group late irradiation (IRT): placement of implants surgery and irradiation after 4 weeks; d) Group Irradiation (Ir): irradiation and after 4 weeks of surgery implant placement. The animal IRI groups, IRT and Go will be subjected to irradiation in a single step in dose of 30 Gy. Plasma levels of TNF-Q, IL-1², M-CSF and IL-10 will be measured prior to the time of euthanasia. In periods of 3 days, 2 weeks and 6 weeks after treatment, 06 animals will be euthanized at random. At parts containing the implants are subjected to immunostaining using the antiTRAP, osteocalcin and p PARP-85 markers. All data will be statistically analyzed initially by the normality test towards the implementation of the appropriate statistical test. The significance level It is the conventional value of 5% (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VEGIAN, MARIANA RAQUEL DA CRUZ; COSTA, BRUNO CESAR ALMEIDA; SANTANA-MELO, GABRIELA DE FATIMA; GODOI, FERNANDA HERRERA COSTA; KAMINAGAKURA, ESTELA; TANGO, RUBENS NISIE; DO PRADO, RENATA FALCHETE; DE OLIVEIRA, LUCIANE DIAS; FEDERICO, CLAUDIO ANTONIO; AVELINO, SARAH DE OLIVEIRA MARCO; et al. Systemic and local effects of radiotherapy: an experimental study on implants placed in rats. CLINICAL ORAL INVESTIGATIONS, v. 24, n. 2, p. 785-797, FEB 2020. (17/04389-0, 17/00543-5, 16/19707-5, 15/24986-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VEGIAN, Mariana Raquel da Cruz. Modulating effect of ionizing radiation on blood cytokines and bone tissue cells at the bone-implant interface. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.