EFFECT OF TRANSPLANTATION OF POLYCAPROLACTONE TUBE (PCL) COATED WITH MESENCHYMAL STEM CELLS IN PERIPHERAL NERVE REGENERATION IN RATS

• Grant number: 16/14364-2
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2017
• Effective date (End): October 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal researcher: Rogério Martins Amorim
• Grantee: Diego Noé Rodríguez Sánchez
• Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Functional recovery after complete peripheral nerve transection is poor and incomplete, occurring axonal degeneration, wich is characterized by myelin fragmentation and separation. Although the peripheral nervous system presents an inherent capacity for regeneration, severe injuries do not allow nerve stumps reconnection. For proper nerve regeneration is essential to maintain a permissive microenvironment. In this context, cell constituents, geometrical organization of components and neurotrophic factors has an essential role for peripheral nerve regeneration. The tubulation techniques allow growth of nerve fibers. Although the introduction of new microsurgical techniques remains, this process is rarely satisfactory for the neuroregeneration and nerve functional recovery. Cell therapy, especially with multipotent mesenchymal stem cells (MSC), has assumed the focus by neurogenerative, anti-inflammatory and immunomodulatory properties. In addition to the human species, canine species also has a high incidence of traumatic lesions of peripheral nerves and may be used as a natural experimental model. This study aims to compare the effects of canine and murine adipose-derived MSC seeded in polycaprolactone tube (PCL) wall, in the regeneration of the experimental model of the sciatic nerve neurotmesis. Our hypothesis is to determinate if the combined use of a PCL tube and MSC in the transected nerves stimulates neuregeneration and functional recovery, improving functional, electrophysiological, morphological aspects and expression of cytokines and neurotrophic factors.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUEZ-SANCHEZ, DIEGO NOE; ARAUJO PINTO, GIOVANA BOFF; CARTAROZZI, LUCIANA POLITTI; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE; CARVALHO BOVOLATO, ANA LIVIA; DE CARVALHO, MARCIO; LOPES DA SILVA, JORGE VICENTE; DERNOWSEK, JANAINA DE ANDREA; GOLIM, MARJORIE; BARRAVIERA, BENEDITO; FERREIRA, RUI SEABRA; DEFFUNE, ELENICE; BERTANHA, MATHUES; AMORIM, ROGERIO MARTINS. 3D-printed nerve guidance conduits multi-functionalized with canine multipotent mesenchymal stromal cells promote neuroregeneration after sciatic nerve injury in rats. STEM CELL RESEARCH & THERAPY, v. 12, n. 1 MAY 29 2021. Web of Science Citations: 1.

RODRIGUEZ SANCHEZ, DIEGO NOE; DE LIMA RESENDE, LUIZ ANTONIO; ARAUJO PINTO, GIOVANA BOFF; DE CARVALHO BOVOLATO, ANA LIVIA; POSSEBON, FABIO SOSSAI; DEFFUNE, ELENICE; AMORIM, ROGERIO MARTINS. Canine Adipose-Derived Mesenchymal Stromal Cells Enhance Neuroregeneration in a Rat Model of Sciatic Nerve Crush Injury. CELL TRANSPLANTATION, v. 28, n. 1, p. 47-54, JAN 2019. Web of Science Citations: 2.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SÁNCHEZ, Diego Noé Rodríguez. Nerve guidance channels functionalized with multipotent mesenchymal stromal cells promotes sciatic nerve renegeration in rats. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.