Effect of chronic and exclusive treatment with aldosterone on the CD3 and CD68 cells infiltration in renal tissues of rats

• Grant number: 16/20459-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2017
• End date: January 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Deise Carla Almeida Leite Dellova
• Grantee: Lucca Denucci Zanini
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

Aldosterone is a steroid hormone that plays an important role in body fluid and electrolyte homeostasis. Studies in models of hypertension, induced by aldosterone and sodium treatment, showed renal and cardiovascular inflammation. However, such experiments did not show the influence that chronic and exclusive treatment with aldosterone (no supplementation with NaCl) has on kidney inflammation (a change that contributes to tissue remodeling, due to fibrosis induction). Thus, this project aims to detect the presence of CD3 (lymphocytes) and CD68 (macrophages) cells by immunohistochemical staining in rat (Wistar, male) kidney (cortical and medullary regions), after treatment with aldosterone (150 µg / kg) for 21 days. It will also be evaluated the mineralocorticoid receptor (MR) antagonism on the inflammatory infiltration, by treatment with an aldosterone and / or spironolactone (20 mg / kg / 21 days). In order to clarify the aldosterone importance on the inflammatory and fibrotic renal processes it becomes relevant the confirmation of lymphocyte (CD3) and macrophages (CD68 or ED1) infiltration in kidney of rats treated chronic and exclusively with aldosterone. (AU)