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Clinical evaluation of bovines exposed to high concentration of heavy metals and its relationship with intestinal, ruminal and respiratory microbiota change and the development of antibiotic resistance in bacteria

Grant number: 16/23204-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2017
Effective date (End): January 01, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Lilian Gregory
Grantee:Natália Carrillo Gaeta
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):19/06932-9 - Metagenomic characterization of the gut, ruminal, and respiratory microbiota of dairy cattle in a heavy metal contaminated environment., BE.EP.DR


Heavy metals are considered the most dangerous polutents to the environment, and to the human being. They usually are the subject of studies about the environmental impacts. Mineral exploration is responsible for the production of high volumes of toxic residues. Mariana city was severely affected by the deposition of residues resulting from the mineral exploration after the rupture of the dam of Fundão. High concentrations of heavy metals can, through several mechanisms, lead to antibiotic resistance, modification of the ruminal, intestinal and possibly respiratory microbiota, impacting animal health and, consequently, the economy of Mariana. The aim of the present study was to evaluate the clinical performance of cattle exposed to high concentrations of heavy metals and to evaluate the impact on bacterial resistance to antimicrobials as well as study possible changes in the respiratory, intestinal and ruminal microbiota of cattle raised in the Rio Doce, Minas Gerais. 30 exposed animals and 30 animals not exposed to high concentration of heavy metals raised in the region and in regions near Mariana, Minas Gerais, will be physically evaluated. Samples of hepatic tissue, whole blood (to obtain serum), fecal swab and deep nasal swab, rumen fluid and milk will be collected. Renal and hepatic profile as well as the concentration of heavy metals in the serum will be determined; The cell conformation as well as the concentration of the metals in the hepatic tissue; intestinal, ruminal and respiratory microbiomes and, finally, the presence of bacterial resistance to antimicrobials in the samples of milk and feces will be performed.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VAN SYOC, EMILY; CARRILLO GAETA, NATALIA; GANDA, ERIKA. Choice of Commercial DNA Extraction Method Does Not Affect 16S Sequencing Outcomes in Cloacal Swabs. ANIMALS, v. 11, n. 5 MAY 2021. Web of Science Citations: 0.
GAETA, NATALIA CARRILLO; BEAN, EMILY; MILES, ASHA MARIE; CARVALHO, DANIEL UBRIACO OLIVEIRA GONCALVES DE; ALEMAN, MARIO AUGUSTO REYES; CARVALHO, JEFERSON SILVA; GREGORY, LILIAN; GANDA, ERIKA. A Cross-Sectional Study of Dairy Cattle Metagenomes Reveals Increased Antimicrobial Resistance in Animals Farmed in a Heavy Metal Contaminated Environment. FRONTIERS IN MICROBIOLOGY, v. 11, NOV 16 2020. Web of Science Citations: 0.
LILIAN GREGORY; NATÁLIA CARRILLO GAETA; ASTRID BETTINI; MARCO CEOL; ALEXANDER TAVELLA. Use of a commercial ELISA kit specific for glycoprotein E peptides to indirectly detect Caprine Herpesvirus 1 (CpHV-1) in the state of São Paulo, Brazil. Arq. Inst. Biol., v. 87, p. -, 2020.

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