Cortisol influence on lutein cells response to insulin stimulation in bitches

• Grant number: 16/22589-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2017
• End date: October 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Paula de Carvalho Papa Keohane
• Grantee: Arnaldo Shindi Maruyama
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 14/00739-9 - Insulin signaling in bovine and canine corpus luteum under the influence of high and low 17²-estradiol concentrations, AP.TEM

Abstract

In previous studies it was shown that luteinic cells respond to insulin stimulation by increasing glucose intake as well as in adipose and muscular cells. The present study aims to analyze cortisol (stress-related hormone) influence on luteinic cells response when stimulated by insulin. For this, we will characterize the gene expression in cells treated or not treated with cortisol. We hypothesize that cortisol has effects contrary to insulin on glucose intake. In this project, 20 bitches will be followed during two diestrous. Fecal and saliva samples will be collected in the first diestrous in order to determine saliva cortisol and fecal glucocorticoid metabolites (FGM) profiles during this period. The same bitches will be submitted to ovariosalpingohisterectomy (OSH) during the second diestrous and the corpus luteum will be collected. In vitro cell culture of these samples will be performed. Hereafter the cells cultures will be stimulated with insulin and cortisol (CI group), only insulin (I0 group), only cortisol (C0 group) or there will be no stimuli (00 group). Afterwards real time PCR will be performed on these samples for the purpose of evaluation of the differentially expressed genes under distinct conditions. Data will be statistically analyzed through GraphPad Prism 5 program with significance level p < 0,05. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARUYAMA, Arnaldo Shindi. Cortisol profile and glucocorticoid signaling pathways in canine corpus luteum. 2018. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.