Rheological aspects and determination of residual DNA in decellularized trachea using multifunctional equipment

• Grant number: 16/25204-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2017
• End date: December 31, 2017
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Elenice Deffune
• Grantee: Juliana Kitagawa Martins
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The trachea presents a cylindrical structure and is extremely important for the breathing process, being able to stretch during an inspiration, contract in the exhalation and eliminate foreign particles whenever necessary. It has many layers that together have stiffness and functionality that are required. Thus, a fabrication of a trachea through functional tissue engineering for grafting, while promising, it is very challenging, for it is a complex organ with important components including cartilage, epithelium and vasculature. The best alternative is the use of scaffolds derived from decellularized trachea without interfering with extracellular matrix properties (ECM), providing structural support without producing inflammation, as well as a regenerative microenvironment without the need for immunosuppression. But in order for the scaffold to meet the requirements, an adequate evaluation of its training must be carried out. The objective of this work is to evaluate the tensile strength, resistance and quantity of residual DNA of decellularized trachea using multifunctional device combining techniques of photonics, ultrasound, chemical bath and mechanical processes. (AU)